不同方法制备冷沉淀的质量评估  

Evaluation on Quality of Cryoprecipitate Prepared from Fresh Frozen Plasma with Different Methods

作　　者：李雪梅[1] 鲍自谦[1] 伍秀英[1] 邬旭群[1] 

机构地区：[1]深圳市血液中心,广东深圳518035

出　　处：《现代检验医学杂志》2006年第2期18-19,共2页Journal of Modern Laboratory Medicine

摘　　要：目的通过对poo l法和水浴虹吸法制备冷沉淀的质量评估,选择合适的制备冷沉淀的方法。方法利用血凝仪检测冷沉淀中FⅧ和Fg的含量,并计算FⅧ和Fg的合格率和冷沉淀的容量。结果poo l法和水浴虹吸法的FⅧ含量(IU/袋)分别为:54.47±25.65,96.50±27.63,FⅧ合格率分别为:26.25%,83.75%,两者均有显著性差异(P<0.001);Fg的含量无显著性差异(P>0.05),Fg合格率均为100%;poo l法制备的冷沉淀的容量明显高于水浴虹吸法(P<0.001)。结论水浴虹吸法无论在方法学上和制备冷沉淀的质量上均明显优于poo l法。Objective To evaluate quality of cryoprecipitates prepared from fresh frozen plasma with pool＇s method and siphonage method thawing at 4 ℃ water bath, and to select properly method for preparing cryoprecipitates. Methods The blood coagulometer was used to detect factor Ⅶ （FⅦ） and fibrinogen （Fg） in cryoprecipitates, and to calculate qualified yield and capacity of cryoprecipitates. Results Concentrations of FⅦ in cryoprecipitates prepared with pool＇s method and siphonage method were 54. 47±25.65（IU/pack）,96.50±27. 63（IU/pack）, and the qualified yield of FⅦ was 26. 65 %, 83. 75 % respectively. There were significant difference in FⅦ concentration and qualified yield （P〈0. 001 ）, but were no difference in Fg concentrations between pool＇s method and siphonage method （P〉0. 05）. All qualified yield of Fg were 100% in the two methods, cryoprecipitates prepared with pool＇s method had much more capacity than simpon method （P 〈0. 001）. Conclusion Siphonage method will be more preferable than pool＇s method in methodology and quality of cryoprecipitates.

关 键 词：冷沉淀 凝血因子Ⅷ 纤维蛋白原 质量 

分 类 号：R457.12[医药卫生—治疗学]