大通牦牛三个功能基因部分序列的PCR-RFLP研究  

Study on PCR-RFLP of part sequence of three functionality gene from Datong Yark

作　　者：白文林[1] 郑玉才[2] 尹荣焕[1] 窦全林[3] 颜卉[1] 罗光彬[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,辽宁沈阳110161 [2]西南民族大学生命科学与技术学院,四川成都610041 [3]青海大学农牧学院,青海西宁810003

出　　处：《黑龙江畜牧兽医》2006年第4期13-15,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金资助项目(39870607)

摘　　要：采用PCR-RFLP技术,检测了大通牦牛α_(S1)-酪蛋白(α_(S1)-casein,α_(S1)-CN)基因、κ酪蛋白(κ-casein,κ-CN)基因和生长激素(growth hormone,GH)基因部分序列的遗传多态性。结果表明大通牦牛群体中α_(S1)-CN基因PCR-RFLP位点呈单态;κ-CN基因和GH基因2个PCR-RFLP位点均呈多态性。κ-CN基因PCR-RFLP位点等位基因A和B的基因频率分别为0.1117和0.8883;GH基因PCR-RFLP位点等位基因A和B的基因频率分别为0.1755和0.8255。适合性卡方检验结果表明,大通牦牛GH基因和κ-CN基因PCR-RFLP位点的基因型分布均极显著偏离Hardy-Weinberg平衡定理(P<0.01);该3个位点平均基因一致度和基因多样度分别为0.8374和0.1626。By the technology of polymerase chain reaction - restriction fragment length polymorphism （ PCR - RFLP） , genetic polymorphism of part sequence of αS1- - casein gene, κ- casein Gene and growth hormone gene from Datong yak was tested. The result showed ： the PCR - RFLP site of αS1- - casein gene was monomorphic, and the PCR - RFLP sites of κ- casein Gene and growth hormone gene from Datong yak were polymorphic. The allele frequencies of A and B were 0. 111 7 and 0. 888 3 of κ- casein gene PCR - RFLP respectively, and the allele frequencies of A and B were 0. 175 5 and 0. 825 5 of growth hormone gene PCR- RFLP respectively; The genotype distribution of κ- casein Gene and growth hormone gene PCR- RFLP sites from Datong yak didnt fit significantly Hardy - Weinberg Law（ P 〈 0. 01 ）. The average gene homozygosity and diversity were 0. 837 4 and 0.162 6 respectively in Datong yak.

关 键 词：大通牦牛 αS1-酪蛋白基因 Κ-酪蛋白基因 生长激素基因 多态性 

分 类 号：S823.85[农业科学—畜牧学]