反复热性惊厥过程中γ-氨基丁酸B受体对一氧化氮／一氧化氮合酶体系的调节作用  

Effect of gamma-aminobutyric acid B receptor on nitric oxide/nitric oxide synthase system during recurrent febrile seizures

作　　者：韩颖[1] 秦炯[1] 卜定方[2] 杨志仙[1] 常杏芝[1] 杜军保[1] 

机构地区：[1]北京大学第一医院儿科 [2]北京大学第一医院中心实验室,北京100034

出　　处：《北京大学学报（医学版）》2006年第2期132-134,共3页Journal of Peking University:Health Sciences

基　　金：卫生部临床学科重点项目(20010912)资助~~

摘　　要：目的:探讨γ-氨基丁酸B受体(γ-aminobutyric acid B receptor,GABABR)对热性惊厥(febrile seizure,FS) 大鼠一氧化氮(nitric oxide,NO)／一氧化氮合酶(nitric oxide synthase,NOS)体系表达的影响。方法:将21 d龄SD 大鼠随机分为对照组、FS组、FS+巴氯芬(baclofen)组和FS+法克罗芬(phaclofen)组。采用热水浴诱导大鼠FS, 隔日诱导1次,共10次。采用分光光度计法测定大鼠血浆中NO含量;用原位杂交方法观察神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)mRNA表达情况;用免疫组化方法观察nNOS蛋白表达情况。结果:FS+ baclofen组NO含量低于FS组[(19．02±9．31)μmol／L比(40．03±9．12)μmol／L],同时nNOS蛋白和mRNA表达也较FS组减弱;而FS+phaclofen组NO含量高于FS组[(66．46±8．15)μmol／L比(40．03±9．12)μmol／L],同时nNOS蛋白和mRNA表达也较FS组增强。结论:反复热性惊厥过程中,GABABR的改变可影响NO／NOS体系的表达。Objective：To explore the effect of γ-aminobutyric acid B receptor（ GABABR） on nitric oxide （ NO）/nitric oxide synthase（NOS） system during recurrent febrile seizures （FS）. Methods： Sprague-Dawley rats aged 21 days were randomly divided into four groups： control group, FS group, FS ＋ baclofen group, FS＋ phaclofen group. FS in rats were induced ten times in a bath of warm water, once every 2 days. The plasma level of NO was detected by the spectrophotometer; the expression of nNOS mRNA was examined by in situ hybridization; the expression of nNOS protein was observed by immunohistochemistry. Results：Compared with those in FS group, the plasma level of NO decreased [ （19.02 ±9.31 ） μmol/L vs （40.03 ±9. 12） μmol/L, and the expression of nNOS was down-regulated in FS ± baclofen group; the plasma level of NO increased [（66.46 ± 8. 15） μmol/L vs （40.03 ±9. 12） μmol/L and the expression of nNOS was up-regulated in FS ＋ phaclofen group. Conlusionp GABABR modulated the expression of NO/NOS system during recurrent FS.

关 键 词：惊厥 发热性 Γ-氨基丁酸 受体 GABA-A 一氧化氮 一氧化氮合酶 

分 类 号：R720.597[医药卫生—急诊医学]