慢性阻塞性肺病患者血浆及诱导痰中尾加压素Ⅱ的变化及其意义  

Change and clinical implication of urotensin Ⅱ in plasma and induced sputum of chronic obstructive pulmonary disease

作　　者：路明[1] 姚婉贞[1] 陈亚红[1] 丁艳苓[1] 常春[1] 唐朝枢[2] 

机构地区：[1]北京大学第三医院呼吸内科,北京100083 [2]北京大学基础医学院生理学与病理生理学系

出　　处：《北京大学学报（医学版）》2006年第2期155-158,共4页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金(30300143 30440073)资助~~

摘　　要：目的:探讨尾加压素Ⅱ(urotensinⅡ,U-Ⅱ)在慢性阻塞性肺病(chronic obstructive pulmonary disease, COPD)发病中的意义。方法:采用放射免疫法测定34例COPD患者和10例正常健康人血浆及诱导痰中U-Ⅱ含量,并常规进行肺功能检测和诱导痰细胞计数和分类。结果:COPD患者和对照组诱导痰U-Ⅱ含量分别为血浆含量的82倍和65倍(P均<0．01),诱导痰和血浆U-Ⅱ水平无相关性(r=0．168,P>0．05)。COPD患者和健康对照组血浆U-Ⅱ含量差异无统计学意义[1．46(1．15,1．73)vs 1．61(1．31,2．17)μg／L,P>0．05,四分位数间距],而 COPD患者诱导痰U-Ⅱ含量较对照组升高约15％[119．87(105．03,132．60)vs 104．44(56．33,122．24)μg／L,P< 0．05,四分位数间距],并且随着肺功能下降,吸烟量的增加,诱导痰U-Ⅱ含量有逐渐升高趋势。诱导痰U-Ⅱ水平与诱导痰细胞总数和中性粒细胞数呈正相关(分别r=0．454,r=0．431,P均<0．01),与FEV1占预计值百分数和血P(O2)呈负相关(分别r=-0．4383,r=-0．518,P均<0．05)。结论:COPD时气道U-Ⅱ分泌增加,U-Ⅱ可能以旁／自分泌的方式参与COPD气道重塑的发病机制。Objective： To investigate the roles of urotensin Ⅱ （U-Ⅱ ） in chronic obstructive pulmonary disease （COPD）. Methods： Plasma and induced sputum were obtained from thirty four patients with stable COPD and ten healthy volunteers. The levels of U-Ⅱ in plasma and induced sputum were measured by RIA kit. Lung function was performed routinely. Induced sputum cells were counted with hemacytometer and differentiated with Wright-Giemsa stain. Results： The levels of U- Ⅱ in induced sputum were 82 and 65 folds higher than those of plasma U- Ⅱ in COPD patients and healthy controls （ P 〈 0.01 ）. The levels of U- Ⅱ in plasma were unrelated to those of induced sputum（ r =0. 168, P 〉0.05 ）. No difference was noted between COPD and healthy controls in the plasma U- Ⅱ levels [ 1.46 （ 1.15, 1.73） vs 1.61（1.31,2. 17） μg/L, medians with interquartile ranges, P 〉0.05]. Sputum U-Ⅱ levels from COPD patients were 15% higher than those of healthy controls [ 119.87 （105.03,132.60） vs 104.44（56.33,122.24 ） μg/L, medians with interquartile ranges, P 〈 0.05 ]. Induced sputum U- Ⅱ levels of COPD patients had a trend of increase as lung function deteriorated and smoking index increases. Raised sputum total cell and neutrophil counts correlated strongly with the levels of U- Ⅱ in induced sputum（r =0.454, r =0.431 ,both P 〈0.01 ）. The levels of U-Ⅱin induced sputum correlated negatively with FEV1 % predicted and p （ O2 ） （ r = - 0.417, r = - 0.518, both P 〈 0.05 ）. Conclusion： U- Ⅱ may act locally, or, via paracrine or autocrine way, play a role in the mechanism of the airway inflammation and airway remodeling in COPD.

关 键 词：血管升压素类 肺疾病 慢性阻塞性 气管 

分 类 号：R563[医药卫生—呼吸系统]