大黄素对体外培养Cajal间质细胞钙离子振荡功能的影响  

Effects of emodin on the function of Ca^2＋ oscillation of ICC cultured in vitro

作　　者：李春穴[1] 童卫东[1] 刘宝华[1] 龙在云[1] 

机构地区：[1]重庆第三军医大学大坪医院普外科,400042

出　　处：《结直肠肛门外科》2006年第1期2-5,共4页Journal of Colorectal & Anal Surgery

基　　金：国家自然科学基金资助(No.30300156)

摘　　要：目的:探讨大黄素对体外培养Cajal间质细胞钙离子振荡的影响,从而进一步揭示大黄素等促进胃肠动力的分子机制方法:体外培养小鼠ICC,体外添加大黄素,应用钙离子指示剂标记细胞内钙离子含量,在激光共聚焦显微镜下动态观察其钙离子振荡功能的变化。结果:免疫荧光鉴定结果证实体外培养细胞为ICC,体外培养ICC具有钙离子振荡功能,大黄素能显著增强其钙离子振荡的频率和振幅。结论:大黄素能显著增强体外培养ICC钙离子振荡功能,可能是其促进胃肠动力的重要机制。Objective：To study the effects of emodin on the function of Ca^2＋ oscillation in interstitial cells of Cajal （ICC） cultured in vitro. Methods：ICC of murine small intestine were cultured in vitro. The function of Ca^2＋ oscillation associated slow waves before and after adding emodin was detected under confocal microscope. Results： Emodin could significantly increase the frequency （about 34 circles / min） and amplitude of vibration. Conclusion：ICC of murine small intestine cultured in vitro show morphologic characters. Cultured ICC of murine small intestine in vitro have function of Ca^2＋ oscillation linked slow waves and emodin can significantly increase its frequency and amplitude of vibration.

关 键 词：大黄素 CAJAL间质细胞 钙离子振荡 体外培养 

分 类 号：R285.5[医药卫生—中药学]