大鼠系膜增生性肾炎时NF-κB和明胶酶B表达的变化及虫草菌丝的影响  

Expression of NF-kappa B and gelatinase B in renal tissue of mesangial proliferative glomerulonephritis rats and effect of Cordyceps sinensis on it

作　　者：吴涛[1] 柳刚[1] 李学刚[1] 高蕾[1] 亓同刚[1] 关广聚[1] 

机构地区：[1]山东大学第二医院肾内科,山东济南250033

出　　处：《中国药理学与毒理学杂志》2006年第2期102-107,共6页Chinese Journal of Pharmacology and Toxicology

基　　金：山东省医药卫生科技发展计划项目(2005052);山东省中医药科技发展计划项目(2005039)~~

摘　　要：目的探讨NF-κB及明胶酶B在系膜增生性肾小球肾炎发病中的作用及虫草菌丝能否用于治疗。方法60只Wistar雄性大鼠随机分为3组。对照组仅给予生理盐水灌胃,模型组和虫草菌丝组制造系膜增生性肾小球肾炎模型共8周,开始制模10d时,虫草菌丝组灌胃给虫草菌丝生理盐水悬浊液4.95mg·g-1·d-1,共46d。4周及8周末取24h的尿液,记尿量,查尿蛋白、尿红细胞和血肌酐。4周末各组各处死8只,8周后全部处死。取两侧肾脏,分别应用微波修复免疫组化法和实时荧光定量逆转录-聚合酶链反应法检测各组肾组织NF-κB及明胶酶B蛋白质及基因表达水平。结果模型组和虫草菌丝组NF-κB及明胶酶B蛋白质及基因表达水平均明显高于对照组;与模型组相比,虫草菌丝组肾小球、肾小管NF-κB蛋白表达减少〔(49.0±3.7)%vs(68.4±9.2)%〕,〔(39.7±6.8)%vs(78.7±9.0)%〕;肾小球的明胶酶B蛋白表达〔(18.0±2.6)%vs(28.3±8.6)%〕亦减少;肾组织NF-κB和明胶酶B的基因表达也减少。结论NF-κB及明胶酶B的过度表达可能在系膜增生性肾小球肾炎发病中起一定作用;虫草菌丝可以抑制NF-κB活性和调节明胶酶B的表达。AIM To investigate the role of NF- kappa B（NF-κB） and gelatinase B in the pathogenesis of mesangial proliferative glomerulonephritis（MPG） and if Cordyceps sinensis can be used in the therapy of MPG. METHODS Sixty male Wistar rats were randomly divided into three groups. One group received only physiological saline by gavage as controls. The MPG model was prepared in a 8-week course as described in literature. On d 10 after the beginning of model preparation the Cordyceps sinensis group was gavaged with fertilized Cordyceps sinensis dry powder suspended in saline 4.95 mg·g^-1·d^-1 for 46 d. Eight rats of each group were sacrificed at the end of 4th week. The rest were sacrificed at the end of 8th week. The kidneys of each rat were separately harvested. The microwave-based immunohistochemistry and real-time quantitative PCR was performed to detect the expression of NF-κB and gelatinsae B. Twenty-four-hour urine volume, 24-h urine protein, urine erythrocyte and serum creatinine in rats were also examined. RESULTS The expression of NF-κB and gelatinsae B in model group and Cordyceps sinensis group was higher than that of control group. Compared with the model group, the protein expression of NF-κB of Cordyceps sinensis group in glomerulus [（49.0±3.7）% vs （68.4 ±9.2）%3 and renal tubule[（39. 7 ± 6. 8）% vs （78. 7 ± 9.0） %] was lower. The protein expression of gelatinsae B of the Cordyceps sinensis group in glomerulus [ （ 18. 0 ± 2. 6 ） % vs （ 28. 3 ± 8.6） % ） was also lower. Compared with the model group, the mRNA expression of NF-κB and gelatinsae B of the Cordyceps sinensis group in nephritis tissue was lower. CONCLUSION Overexpression of NF-κB and gelatinsae B may play an important role in the pathogenesis of MPG. The Cordyceps sinensis can restrain the activity of NF-κB and adjust the expression of the gelatinase B.

关 键 词：NF-ΚB 明胶酶B 系膜增生性肾小球肾炎 虫草菌丝 

分 类 号：R285.5[医药卫生—中药学]