同种异体胚脑移植免疫排斥反应实验研究  

Experimental research on immunological rejection in embryonic brain allograft

作　　者：曾水林[1] 韩洋[1] 王磊[1] 雷志年[1] 朱建宝[1] 李涛[1] 

机构地区：[1]东南大学神经生物学研究所,江苏南京210009

出　　处：《中华神经外科疾病研究杂志》2006年第2期127-131,共5页Chinese Journal of Neurosurgical Disease Research

摘　　要：目的观察同种异体胚脑组织脑内移植是否存在免疫排斥反应以及免疫抑制剂治疗对移植物存活的影响。方法将孕14.5d胚鼠腹侧中脑(VM)细胞悬液移植到帕金森病(PD)模型大鼠纹状体内,术后部分动物每天给予甲基强的松龙(MP)腹腔注射(30mg/kg),分别存活10d、21d、35d和60d,经旋转行为测试后分批处死,行CD4、CD8、主要组织相容性复合体Ⅱ类分子(MHC-Ⅱ)和酪氨酸羟化酶(TH)免疫组化染色。结果移植组10d、21d时,CD4+、CD8+和MHC-Ⅱ+细胞的数量较对照组明显增多,21d时更为显著,35d时部分移植区出现排斥反应,60d时移植区未见移植组织存活。MP治疗组在10d、21d时,移植区CD4+、CD8+和MHC-Ⅱ+细胞数量较未治疗组明显减少,35d时未见明显排斥反应;60d时,CD4+、CD8+和MHC-Ⅱ+细胞的数量较35d显著增加,部分移植物被排斥。MP治疗组各时间点TH+细胞数量明显多于非治疗组,但大鼠的旋转行为在各时间点均未见改善。假手术组在10d、21d时,见少量CD4+、CD8+和MHC-Ⅱ+细胞,35d时消失,各时间点都未见TH+神经元。结论同种异体胚脑组织脑内移植存在免疫排斥反应。MP治疗在一定程度上可以减弱或延缓免疫排斥反应的发生。Objective To investigate the occurrence of rejection in embryonic brain allograft and the effects of immunosuppressive treatment on the survival of neural grafts. Methods Single cell suspensions were derived from the brain of embryonic 14.5 d SD rat and were allografted into the striatum of the model of Parkinson＇s disease recipient rats. Some animals were given daily injection of methylprednisolone （IMP） 30 mg/kg i. p after grafting. Surviving animal were sacrificed at 10 d , 21 d , 35 d and 60 d after transplantation, and the expression of tyrosine hydmxylase （TH）, CD4, CD8 and MHC-Ⅱ antigen in brain tissue were detected by immunocytochemical staining.Results Graft group elicited high CD4, CD8 and major histocompatibility complex （MHC）-Ⅱ expressing within and around the allngrafts compared with control group at 10 d, 21 d after grafting. Some grafts had not survived at 35 d and 60 d. Compared with non-MP treatment groups, the numbers of CJ）4, CD8 and MHC-Ⅱ immunopositive cells in all MP treatment groups were significantly low, but the expression of TH was high in MP treatment groups at 10 d, 21 d. There was no remarkable rejection evidence at 35 d. But the numbers of CD4, CD8 and MHC-Ⅱ immunopositive cells increased after 60 d in MP treatment groups. There was no significant difference in the rotation behavior after transplantation.Conclusion The brain is not an ＂immunologically privileged site＂ and there is rejection existing in embryonic brain allograft. Immunosuppressive treatment does not sufficiently protect from immunolngical rejection, but it is necessary for long-term graft survival and functional recovery.

关 键 词：胚脑 同种异体移植 免疫排斥 甲基强的松龙 帕金森病 

分 类 号：R392[医药卫生—免疫学]