肌肉异位表达pGHRH的两种质粒对大鼠的促生长作用  

Two Kinds of pGHRH Myogenic-expression Plasmids for Enhancing Rat Growth

作　　者：孟庆勇[1] 张雅丽[2] 解启发[1] 巩霞[1] 李宁[1] 

机构地区：[1]中国农业大学生物学院生物技术国家重点实验室,北京100094 [2]中国农业大学食品学院,北京100094

出　　处：《生物化学与生物物理进展》2006年第4期357-361,共5页Progress In Biochemistry and Biophysics

基　　金：国家重点基础研究发展规划资助项目(G20000161).

摘　　要：构建了两种猪生长激素释放激素(GHRH)的肌肉特异表达质粒ppG-A53f-H6-GHRH(ppG-H6)及ppG-A53f-H4-GHRH(ppG-H4).通过对大鼠进行肌肉注射,并于注射后分别记录0,3,7,11,14,17,20,24,27,31,34,38以及第41天的体重变化结果,同时分析了第20、27、34和第41天的血清生长激素(GH)浓度,以研究促生长表达质粒在动物体内的促生长作用.通过研究表明,外源的GHRH表达质粒在肌肉中获得了表达,并使动物体内的GH水平维持在一个相对稳定的较高水平上,导致的直观效果表现为:通过肌肉注射表达质粒ppG-H6对大鼠的生长有促进效果,在第34天的净增重显著于对照组((200.57±3.99)gvs(185.85±9.45)g,P<0.05).实验结果预示,利用注射肌肉特异异位表达GHRH蛋白可能促进动物生长.Two kinds of ectopic expression of porcine growth hormone-releasing hormone （GHRH） plasmids, ppG-A53f-H6-GHRH （ppG-H6） and ppG-A53f-H4-GHRH （ppG-H4）, were constructed by using a myogenic expression vector which has α-actin expression elements. After the animal was injected in the muscle with the two plasmids, the body-weight data at the 0 / 3/7/11/ 14/17/20/24/27/31/34/38/41st days were collected. Rat plasma from blood collected at the 20/ 27/ 34/41st days was made and growth hormone （GH） levels were determined by radio immunoassay （RIA）. The results indicated that ppG-H6 and ppG-H4 do enhance the rat growth compared to the control, with the ppG-H6 treated group showing a more effective response. Compared with the control group, at the 34th day after injection, the weight gain of the ppG-H6 group was significant （（200.57±3.99） g vs （185.85±9.45） g, P 〈 0.05）. Furthermore, the GH maintains on a stable level in group ppG-H6 and ppG-H4 compare to control by the end of experiment, which support that the injection of ppG-H6 and ppG-H4 plasmids caused the body-weight of rat increasing. The results imply that injection of ectopic expression of porcine growth hormone-releasing hormone （GHRH） plasmids is a possible way to enhancing animal growth.

关 键 词：生长激素释放激素 肌肉表达 促生长 质粒 基因治疗 

分 类 号：Q789[生物学—分子生物学]