小鼠和鸡的类E1酶新基因UBAL的克隆和表达分析  

Molecular Cloning and Expression Analysis of a Novel E1 Like Gene UBAL in Mouse and Chicken

作　　者：薛鹏[1] 慈宏亮[1] 陈微 李亦平[1] 

机构地区：[1]北京师范大学生命科学学院生物医学研究所 [2]Forsyth Institute,Harvard School of Dental Medicine,Boston02115,USA

出　　处：《生物化学与生物物理进展》2006年第4期382-387,共6页Progress In Biochemistry and Biophysics

基　　金：浙江赛尔生物医学研究院资助项目.

摘　　要：根据含有UBA_NADbindingdomain的EST序列,利用同源性序列查找和EST拼接的方法,克隆得到鼠、鸡的新基因UBAL(ubiquitin-activatingenzymelikeprotein),它们都具有泛素活化酶Uba1的保守结构域Ⅰ,可能的ATP结合序列GVGGVG和Cys活性位点.用半定量RT-PCR分析11种成年小鼠组织的mUBAL表达量,显示mUBAL在成年小鼠多种组织中都有表达,在肾、睾丸、脑、心脏中尤为丰富.用mUBAL的编码区为探针进行的小鼠胚胎整体原位杂交显示,mUBAL在胚胎发育早期即开始表达,并随着前内脏内胚层(AVE)的向前迁移而迁移,随后在胚胎的端脑区特异性表达.与小鼠胚胎的表达谱相类似,gUBAL在HH14、HH16和HH18期鸡胚中的表达主要集中在头部.根据mUBAL和gUBAL的序列相似性保守结构域和活性位点,可以初步断定它们可能是类泛素活化酶E1的新成员,可能通过活化类泛素蛋白参与胚胎脑部的发育和调控成体组织的功能.According the EST sequence contained a UBA_NAD binding domain, a novel gene UBAL （ubiquitin-activating enzyme like protein） was cloning both in mouse and chicken by the homology scanning and EST splicing methods. UBAL contains the conserved domain I of Ubal, ATP binding motif and putative active site Cys. The expression profile of mUBAL was detected in most of the tissues of the 11 investigated tissues, especially in kidney, testis, brain and heart. Whole-mount in situ hybridization with the coding region of mUBAL showed that expression of mUBAL was presented in the early streak stage of mouse embryo and migrated anterior with anterior visceral endoderm （AVE） and then in the telencephalon. The gUBAL expression was also found in the brain of HH14, HH 16 and HH18 chick embryos. The conserved structures and expression patterns of UBAL suggested that UBAL may play important roles in the developing brain of embryo and adult tissues as E 1 like enzyme.

关 键 词：类泛素蛋白 类泛素蛋白活化酶(UBAL) 克隆 表达谱 

分 类 号：Q785[生物学—分子生物学] Q786