自制磷酸钙骨水泥缓释体中顺铂的体外缓释效能  

In vitro release efficacy of Cisplatin in the self-made calcium phosphate cement

作　　者：黄波[1] 范清宇[1] 潘朝晖[1] 刘云燕[1] 

机构地区：[1]解放军第四军医大学唐都医院全军骨肿瘤研究所,陕西省西安市710038

出　　处：《中国临床康复》2006年第17期56-58,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察自制磷酸钙骨水泥缓释体对顺铂的体外药物缓释性能及其浸泡液对骨肉瘤细胞OS-9607的抑制能力。方法:实验于2004-09/2005-06在解放军第四军医大学唐都医院全军骨肿瘤研究所完成。①磷酸钙骨水泥的制备:将无水磷酸氢钙与碳酸钙按比例混合,1500℃高温煅烧6h形成磷酸四钙,和磷酸氢钙混合制成磷酸钙骨水泥固相成分;配置5%明胶溶液作为液相。②磷酸钙骨水泥缓释体的制备:按95mg磷酸钙骨水泥固相配5mg顺铂的配比,混合后加入液相制成直径10mm、厚3mm的圆片状样品,共5片,每片平均重1150mg,平均每片含顺铂50mg。同法制备不含顺铂的磷酸钙骨水泥样品3片作为阴性对照。③体外释放和体外抑制骨肉瘤细胞实验:随机抽取3片含顺铂样品及阴性对照,各置于0.1mol/L(pH7.4)的磷酸盐缓冲液中,37℃恒温振荡器保存,分别于第1,2,3,5,7,14,28,42,56天取浸泡液标本,用原子吸收分光光度计检测顺铂的释放浓度。四甲基偶氮唑蓝法检测经磷酸钙骨水泥缓释体浸泡液作用后的骨肉瘤细胞OS-9607的活力及代谢活性的变化。结果:①磷酸钙骨水泥缓释体的体外顺铂释放检测结果:浸泡前2d顺铂出现快速释放,分别占总量的21.62%和8.87%,以后在较低水平维持相对稳定的缓慢释放,释放时间持续56d以上。②磷酸钙骨水泥缓释体不同浸泡时间浸出液的顺铂含量及对骨肉瘤细胞OS-9607的抑制率:浸泡第1,14,28,56天的浸出液的顺铂含量分别为2.702,0.081,0.063,0.051g/L,对骨肉瘤细胞OS-9607的抑制率分别为67.78%,48.89%,35.56%和27.78%。结论:在模拟体内环境下,磷酸钙骨水泥对抗肿瘤药物顺铂有缓慢而持久的体外释放作用,且对骨肉瘤细胞OS-9607具有中等抑制效果。AIM：To investigate the sustained release character of Cisplatin impregnated self-made calcium phosphate cement （CPC） in vitro and its inhibitory ability on human osteogenic sarcoma cell OS-9607. METHODS： This experiment was conducted at the Institute of Orthopaedic Oncology, Tangdu Hospital, the Fourth Military Medical Univeraity of Chinese PLA from September 2004 to June 2005.①Preparation of CPC： Tetracalcium Phesphate（TTCP） was made by calcinating the mixture of Calcium Phosphate Dibasic Anhydrous and calcium carbonate at 1 500 ℃ for 6 hours. CPC solid phase was got by mixing Tetracalcium phosphate with calcium phosphate dibasic. 5% gelatin solution was made as its liquid phase. ②Preparation of CPC： The test sample was made by adding CPC/ Cisplatin （95 mg CPC solid phase to 5 mg Cisplatin） into liquid phase. It was round piece-shaped sample with 10 mm in diameter and 3 mm in thickness. There were 5 pieces in total and 1 150 mg on average and 50 mg on average of Cisplatin in each one. 3 other samples without Cisplatin were made as control.③ Experiment of in vitro release and inhibiting osteogenic sarcoma cell in vitro ： 3 pieces of samples with Cisplatin and negative control samples were immerged into 0.1mol/L phosphate buffer solution, the samples were stored at 37 ℃ .The solution.including Cisplatin released from CPC/Cisplatin compound was extracted after 1, 2, 3, 5, 7, 14, 28, 42, 56 days respectively. The extracted solution was analyzed by atomic absorption spectrophotometer （AAS）. The activity of human osteogenic sarcoma Cell OS-9607 was detected with MTr after treated by the soak of CPC, and the change of metabolic activity was also detected. RESULTS： ①In vitro release efficacy of cisplatin of CPC： Cisplatin had been released rapidly during the 1^st to 2^rd day, whose proportion was 21.62% and 8.87% , respectively. Later, CPC kept slowly stable low drug release rate and could exist more than 56 days. ②Content of Cisplatin of CPC at different soaked time and its

关 键 词：磷酸钙类 治疗应用 顺铂 释放动力学 骨肿瘤 

分 类 号：R581[医药卫生—内分泌]