九种常用杀虫剂对二化螟线粒体ATPase活力的抑制作用  

Inhibition of ATPase activity in mitochondria of Chilo suppressalis by nine common insecticides

作　　者：李海平[1,2] 冯涛[1] 陶岭梅[3] 刘学[3] 姜辉[3] 林荣华[3] 梁沛[3] 高希武[1] 

机构地区：[1]中国农业大学昆虫学系 [2]内蒙古农业大学农学院,呼和浩特010019 [3]农业部农药检定所

出　　处：《昆虫学报》2006年第2期254-259,共6页Acta Entomologica Sinica

基　　金：国家重点基础研究发展规划"973"项目(G2000016207);国家自然科学基金项目(39970496)

摘　　要：研究了二化螟Chilo suppressalis线粒体Na^＋-K^＋-ATPase和Ca^2＋,Mg^2＋．ATPase的生物化学性质以及9种常用杀虫剂对这两种酶活性的影响。结果表明，二化螟线粒体Na^＋-K^＋-ATPase和Ca^2＋-Mg^2＋-ATPase的最适反应条件为pH值7．4，温度37℃。Na^＋-．K^＋-ATPase的米氏常数（Kw）为0．42mmol／L，最大反应速度（Vmax）为302．47nmol／（min·mg）。Ca^2＋-Mg^2＋-ATPase的Km为0．40mmol／L，Vmax为128．04nmol／（min·mg）。药剂浓度为1×10^-4mol／L时，5种菊酯类杀虫剂对离体ATPase活性抑制的顺序为：溴氰菊酯〉联苯菊酯〉百树菊酯〉三氟氯氰菊酯和氟硅菊酯；对二化螟Na^＋-K^＋-ATP8se的抑制率分别为40．12％、39．69％、27．27％、19．49％和18．71％；对Ca^2＋-Mg^2＋-ATPase的抑制率分别为29．27％、23．78％、19．88％、11．64％和14．34％。硫丹对二化螟Na^＋-K^＋-ATPase和Ca^2＋-Mg^2＋-ATPase的抑制率均为17．46％。甲胺磷和呋喃丹对Ca^2＋-Mg^2＋-ATPase的抑制率分别为27．16％和17．42％，对Na^＋-K^＋-ATPase则几乎没有抑制作用。实验结果还表明，在1．6×10^-7～1×10^-4mol／L的浓度范围内，上述9种杀虫剂对二化螟ATPase活性的抑制率存在明显的剂量-效应关系。The characteristics of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase in mitochondria of Chilo suppressalis and inhibition of these two types of ATPases by nine common insecticides, i.e., deltamethrin, bifenthrin, cyfluthrin, cyhalothrin, salifluofen, endosufan, methamidophos, carbofuran and triazophos, were determined. The results indicated that the optimum pH value of Na^＋ -K＆＋ -ATPase was 7.4, and the optimum temperature 37℃ for both Na^＋ -K^＋ -ATPase and Ca^2＋ -Mg^2＋ -ATPase. The Michaelis constant （Kn） of Na^＋ - K^＋ -ATPase hydrolyzing ATP was 0.42 mmol/L, not significantly different from that of Ca^2＋ -Mg^2＋ -ATPase （0.40 mmol/L）. The maximum velocities （ Vmax ） hydrolyzing ATP were 302.47 nmol/（ mg·min） for Na^＋ -K^＋ - ATPase and 128.1M nmol/（mg·min） for Ca^2＋ -Mg^2＋ -ATPase, respectively. When the final concentration of insecticides was 1×10^-4 mol/L, the order of inhibition of to Na^＋ -K^＋ -ATPase by five pyrethroids insecticides was deltamethrin 〉 bifenthrin 〉 cyfluthrin 〉 cyhalothrin and salifluofen, The percentage of inhibition of Na^＋ - K^＋-ATPase activity was 40.12%, 39.69%, 27.27%, 19.49%, and 18.71%, and the percentage of inhibition of Ca^2＋ -Mg^2＋ -ATPase was 29.27%, 23.78%, 19.88%, 11.64% and 14.34% by the above five pyrethroids respectively when the concentration of inhibitors was 1×10^-4 mol/L. The potential of endosufan inhibiting the Na^＋ -K^＋ -ATPase activity was same as its potential inhibiting Ca^2＋ -Mg^2＋ -ATPase activity, andthe percentage of inhibition was 17.46%. Methamidophos and carbofuran possessed the potential of inhibition to Ca^2＋ -Mg^2＋ -ATPase, but no inhibition to Na^＋ -K^＋ -ATPase when their final concentration was 1 × 10^-4 mol/ L. The inhibition of ATPase activity by nine insecticides was increased with the increasing of insecticide concentration from 1.6 × 10^-7 to 1×10^-4 mol/L.

关 键 词：二化螟 腺苷三磷酸酶 杀虫剂 酶活性 抑制率 

分 类 号：Q965[生物学—昆虫学]