腺病毒E4启动子结合蛋白-4(E4BP4)基因在小鼠胚胎着床期间子宫组织中的表达  

UTERINE EXPRESSION OF ADENOVIRUS E4 PROMOTERBINDING PROTEIN 4 (E4BP4)DURING THE EMBRYO IMPLANTATION PERIOD IN MICE

作　　者：王健[1] 黄哲平[2] 杨增明[3] 沈维雄[2] 左嘉客[2] 申庆祥[2] 

机构地区：[1]复旦大学上海医学院,上海200032 [2]上海市计划生育科学研究所,上海200032 [3]东北农业大学生命科学院,黑龙江哈尔滨150030

出　　处：《分子细胞生物学报》2006年第2期123-131,共9页Journal of Molecular Cell Biology

基　　金：国家重点基础研究规划项目(TG1999055903)~~

摘　　要：腺病毒E4启动子结合蛋白-4(E4BP4)是哺乳动物细胞核内的一种碱性亮氨酸拉链(bZIP)型转录因子,参与调控细胞的存活和增殖。前期研究表明,它在孕第5天的小鼠着床位点有明显的高表达。本文分别应用Northem blot、in situ杂交、Western blot和免疫组织化学技术,对E4BP4基因在小鼠妊娠初始期子宫、着床期胚胎着床位点和非着床位点的表达情况进行了研究。观察发现：在小鼠妊娠初始期,E4BP4基因在子宫组织中的表达逐步上调；至胚胎着床期间,其在胚胎着床位点的表达水平进一步提高,并明显高于非着床位点；该基因的表达不依赖于胚胎,人工蜕膜化可诱导其表达：E4BP4 mRNA和E4BP4蛋白分子都主要分布于子宫腔周围的基质细胞和蜕膜细胞。上述结果提示E4BP4基因可能通过促进着床位点基质细胞的增殖和抑制蜕膜细胞的凋亡而参与胚胎着床过程的调控。Adenovirus E4 promoter-binding protein 4 （E4BP4）, a mammalian basic leucine zipper （bZIP） nuclear transcription factor, was identified to be involved in cell survival and proliferation. Previous research data showed that the expression of E4BP4 gene was up-regulated at the implantation sites on day 5 pregnancy of mouse. The aim of the present study was to examine the expression pattern of E4BP4 gene in uterus during pre-implantation period,and at the implantation sites and inter-implantation sites during the implantation process, through the Northern blot, in situ hybridization, Western blot and immunohistochemistry analyses. It was found that,（1） during the pre-implantation period, the expression of E4BP4 gene was gradually increased; （2） its expression was sharply up-regulated at the implantation sites compared with that at the interimplantation sites during the embryo implantation process; （3） the uterine expression of E4BP4 gene was not embryo-dependent, but observed in artificially induced decidulization of pseudopregnant mouse; （4） both E4BP4 mRNA and E4BP4 protein were localized in stromal cells and decidual cells around the uterine lumen. These results indicated that E4BP4 may play a critical role in embryo implantation process by promoting stromal cell proliferation and inhibiting deeidual cell apoptosis.

关 键 词：小鼠 E4BP4基因 子宫 胚胎 着床 

分 类 号：Q344[生物学—遗传学]