一种SARS冠状病毒诊断基因芯片的研制  

The Research and Development of New Diagnostic Gene Chips for SARS Coronavirus

作　　者：赖建华[1] 张智清[2] 余敏[1] 吴新伟[3] 任永富[1] 谭德勇[1] 

机构地区：[1]云南大学生命科学学院生化与分子生物学实验室,昆明650091 [2]中国疾病预防控制中心病毒病预防研究所,北京100052 [3]广州市疾病预防控制中心免疫室,广州510045

出　　处：《生物医学工程学杂志》2006年第2期266-270,共5页Journal of Biomedical Engineering

基　　金：云南省科技厅新药专项基金资助项目(2003XY04)

摘　　要：通过筛选SARS冠状病毒的基因序列,获得其有效检测的基因位点;利用已有的基因芯片技术,研制了一张用于SARS冠状病毒诊断的基因芯片。经临床标本检测验证,该基因芯片的粗符合率达到94.29%;检测灵敏度达到10-2/m l病毒分子,具有很好的实用性。The loci of cDNA sequences for valid diagnosis have been identified through the selection of the genome of SARS coronaries. The gene chips for diagnosing such virus have been developed, based on our owndeveloped technology for manufacturing and application of gene chips. The diagnoses given by such gene chips were consistent well with the reports of clinical laboratories （94.29 %） and the sensitivity reached 10^-2/ml virus molecules. This method is well suited for the clinical use in SARS coronaries diagnosis.

关 键 词：SRAS冠状病毒 基因芯片 诊断 

分 类 号：R346[医药卫生—基础医学]