成年大鼠基底前脑nestin阳性神经元的性别差异  

SEX D IFFERENCES OF NESTIN MM UNOREACT1VE NEURONS IN ADULT RAT BASAL FOREBRA IN

作　　者：郭灵[1,2] 袁群芳[1] 谢瑶[1] 汪华侨[1] 姚志彬[1] 

机构地区：[1]中山大学基础医学院解剖学教研室、脑研究室,广州510080 [2]现在地址：广西医科大学人体解剖学教研室,南宁530021

出　　处：《中国生物学文摘》2006年第3期30-34,共5页Chinese Biological Abstracts

基　　金：国家教委跨世纪人才基金（国教95-004）、广东省千百十人才工程基金（97-002）资助项目

摘　　要：为了探讨成年雌雄大鼠基底前脑nestin阳性神经元的性别差异及其意义，本实验应用免疫组织化学方法对正常成年雌雄SD大鼠及去势2周和4周的雌雄SD大鼠基底前脑的内侧隔核（MS）、斜角带垂直支（vDB）及斜角带水平支（hDB）的nestin阳性神经元的变化进行了比较研究。结果显示：正常雌雄大鼠MS、vDB及hDB的ncstin阳性神经元数十分接近（P〉0.05）：而去睾丸2周大鼠组MS,vDB、hDB的nestin阳性神经元数则明显高于去卵巢2周大鼠组（P〈0.05，P〈0.01）；去睾丸4周大鼠组、vDB、hDB的nestin阳性神经元数依然明显高于去卵巢4周大鼠组（P〈0.05），但两组MS间的nestm阳性神经元数差别不大（P〉0.05）；各组大鼠的nestin阳性神经元的胞体和树突均相似。以上结果表明，正常成年雌雄大鼠基底前脑的nestin阳性神经元数不存在性别差异。无论去势与否，nestin阳性神经元的形态始终不存在性别差异；而去势后nostin阳性神经元数却存在着明显的性别差异。提示基底前脑nestin阳性神经元的性别差异不仅与雌激素和雄激素在雌雄个体中所发挥的不同作用密切相关。而且还可能是促使学习记忆出现性别差异的形态学基础。To explore the sex differences of nestin immunoreactive （nestin-IR） neurons in the adult rat basal forebrain, the changes of nestin-R neurons in themcdial septum （MS）, vertical （vDB） and horizontal （hDB） linbs of the diagonal band of Broca in the basal forebrain were ocmpared between adult intact fomnale and male SD rats, and between ovariectumized female SD rats and castrated SD male rats by meansof inmunohistochemical technique Itwas found that no significant differences in the number of nestin-R neurons haMS, vDB and hDB existed between intact female and male rats However, the numberofnestin-IR neurons in the above three subregbnsofcastinted male rats for2 or 4 weekswas significantly greater than that ofovariectanized female ratswith the exception of the number haMS between castrated male rats and ovarieetan ized fan are rats for 2 weeks No obviaus alteratbn for the san as or dendrites of nestin-R neurons existed in the above three subregbns among intact or ganadeclanized female and male rats The above results show that sex differences of the number of nestin-R in adult basal fnrebtain only exist between ovarieetmaized female rats and castrated male rats, while no sex differences of the num bet of nestin-IR neurons exist between in tact female and male rots No sex difference was observed in the shape of nestin-IR neurons among intact or gonadectxnized female and male rats The present results suggest that sex differences of the number in nestin-IR neurons not only are closely related to the different roles of estrogen and androgen in fomale and male individuals, but also can bemorpholeglcal foundations for sex differences in leaming and memory.

关 键 词：巢蛋白 性别差异 基底前脑 大鼠 

分 类 号：Q189[生物学—神经生物学] Q954.52[生物学—普通生物学]