硅肥和接种纹枯病菌对水稻膜脂过氧化和防御酶活性的影响  

Influences of silicon and inoculation with Rhizoctonia solani on membrane lipid peroxidation and defence enzyme activities in rice leaves

作　　者：张国良[1] 戴其根[2] 张洪程[2] 凌励[3] 刁守雨[2] 

机构地区：[1]淮阴工学院生命科学与化学工程学院,江苏淮安223001 [2]扬州大学农学院,江苏扬州225009 [3]江苏农垦集团,南京210008

出　　处：《扬州大学学报（农业与生命科学版）》2006年第1期49-53,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(30571090);国家"十五"重大科技攻关项目(2002BA516A14;2004BA520A03);江苏省农业三项工程项目[SX(010)2000-012-52];江苏省教育厅项目(04KJD210030);淮阴工学院校级科研资助项目

摘　　要：在群体水培条件下,探讨施硅肥和接种纹枯病菌对水稻叶片丙二醛(M DA)含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性的影响。结果表明:未接菌条件下,硅肥能提高POD、CAT的活性,降低SOD活性,M DA含量略有上升;接菌处理使M DA含量、CAT和POD活性升高,SOD活性降低;接菌和施硅肥处理使SOD活性下降,第4天达最小值,但其活性仍高于接菌未施硅肥处理,其POD、CAT活性第4天达最大值,M DA含量第3、4天达最大值;施硅肥后接菌叶片的上位叶片的SOD、CAT和POD活性与接菌叶片无明显差异。施硅肥能显著降低水稻植株的纹枯病病情指数,提高其对纹枯病的抗病能力,相对防治效果达25.8%。The dynamic changes in malonaldehyde （MDA） content, superoxide dismutase （SOD）, catalase （CAT） and peroxidase （POD） activities in rice leaves with supply of silicon and inoculation with Rhizoctonia solani were studied. With no inoculation of the pathogen, MDA content, POD and CAT activities in Si supplied rice plants were higher than those in Si deprived rice plants, but SOD activity was lower. The inoculation enhanced MDA content, POD and CAT activities but decreased SOD activity, Under the condition of inoculation MDA content in Si supplied rice plants was maximum at 3-4 day, SOD activity was minimum at 4 day, SOD activity in Si supplied rice plants was higher than that in Si deprived rice plants, and POD, CAT activities were maximum at 4 day. No significant differences in SOD, CAT and POD activities were found between the inoculated leaves and the upper leaves when Si was supplied for rice plants, After being inoculated of Rhizoctonia solani, the rice plants supplied with Si had significantly lower disease index with relative control effect of 25.8o/00 compared to those deprived with Si.

关 键 词：水稻 硅 纹枯病菌 膜脂过氧化 防御酶 

分 类 号：S511.062[农业科学—作物学]