槲皮素对NIH-3T3细胞氧化损伤的保护作用  

The protection effect of quercitin on oxidative damage in NIH-3T3 cells

作　　者：宫璀璀 郑玉霞 曹阳 郑乃刚[2] 李璇 刘力源 吴景兰[2] 

机构地区：[1]153医院济南军区检验中心,河南郑州450042 [2]郑州大学分子细胞生物学研究中心,河南郑州450052

出　　处：《实用医药杂志》2006年第4期450-452,共3页Practical Journal of Medicine & Pharmacy

摘　　要：目的探讨槲皮素对NIH-3T3细胞氧化损伤的保护作用。方法取处于对数生长期的3T3细胞,平均分为以下四个实验组。槲皮素前保护组(Q1组):先加含有50μmol/L槲皮素培养液培养24h,再换含有0.5mmol/LH2O2的培养液培养30min。槲皮素后保护组(Q2组):先加含有0.5mmol/LH2O2的培养液培养30min;再换含有50μmol/L槲皮素培养液培养24h;H2O2损伤组(H2组):先加含有0.5mmol/LH2O2的培养液培养30min,再换仅含有10%的胎牛血清的培养液培养24h。对照组(C组):仅加10%胎牛血清的DMEM培养液培养24h。收集并裂解各组细胞,检测细胞内T-AOC、SOD、GSH-Px、GSH、NOS、NO、MDA的变化,应用MTT实验检测3T3细胞的生存率。结果Q1与Q2、H2组相比,Q1组细胞内T-AOC、SOD、GSH-Px、GSH均有增加,NOS、NO无明显改变,MDA减少,细胞存活率明显增加。结论槲皮素可能是通过上调抗氧化酶系活性对3T3细胞的氧化损伤产生保护作用。Objective To explore the protection effect of quercitin（Q）on oxidative damage in NIH-3T3 cells.Methods The NIH-3T3 cells in logarithmic growth phase incubated in DMEM were divided into 4 groups as follows, （1）Q pre-protection group （Q1）： prior to incubation with 0.5mmol/L H2O2 for 30min, the 3T3 ceils were incubated with 50μmol/L quercetin for 24h;（2）Q post-protection group （Q2）：after incubation with 0.5mmol/L H2O2 for 30min, the 3T3 cells were incubated with 50μmol/L quercetin for 24h;（3）H2O2 damage group（H2）： the 3T3 cells were incubated with 0.5mmol/L H2O2 for 30min followed by subsequent incubation with 10% fetal bovine serum（FBS） for 24h;（4）The control group（C）：the 3T3 cells were incubated with DMEM medium supplemented with 10% FBS. The T-AOC, SOD, GSH-Px, GSH, NOS, NO and MDA were detected in the lysate of harvested each group ceils. The 3T3 cell survival rate was detected by MTr assay.Results The levels of T-AOC,SOD,GSH-Px,GSH were enhanced;MDA was decreased and NO,NOS had insignificant change;The 3T3 cell survival rate was promoted markedly. Conclusion The enhanced anti-oxidative effect of quercetin on the 3T3 cells may act through upregulation of a series of anti-oxidative enzymen system.

关 键 词：细胞裂解 抗氧化损伤 槲皮素 H2O2 

分 类 号：R282.71[医药卫生—中药学]