高粱抗蚜基因的遗传分析和SSR标记定位  

Analysis of the resistance gene to the sorghum aphid,Melanaphis sacchari,with SSR marker in Sorghum bicolor

作　　者：常金华[1] 夏雪岩[2] 张丽[1] 李荣改[1] 刘国庆[1] 罗耀武[1] 

机构地区：[1]河北农业大学农学院,河北保定071001 [2]河北省农林科学院,河北石家庄050000

出　　处：《草业学报》2006年第2期113-118,共6页Acta Prataculturae Sinica

基　　金：国家自然科学基金(30170498);河北省自然科学基金(2005000254)资助

摘　　要：高粱蚜是高粱生产中的一种毁灭性害虫,利用植物的固有抗性是防治害虫的最有效途径。经多年杂交选育获得1个对高粱蚜免疫的高粱品种“河农16”,用其作亲本与感蚜品系“千三”进行杂交,对亲本、F1、F2抗蚜性鉴定表明,“河农16”和F1对蚜虫表现高抗,F2抗感分离符合3∶1,该抗蚜性受一对基因控制,表现出显性遗传。用已定位到连锁群上的微卫星标记和分离群体分析法,对抗蚜基因进行了连锁分析,发现1个与抗蚜基因连锁的微卫星标记(SSR标记),与抗蚜基因的遗传距离为8.7 cM。该标记位于第9连锁群上,因而将该基因初步定位于第9连锁群。The sorghum aphid, Melanaphis sacchari is an economically important pest of S. bicolor. An effective means to control the aphid is through the use of resistant cultivars. A high resistance S. bicolor cultivar HN-16 was obtained by hybridation and selection. F1 progenies and F2 segregation derived from HN-16 and QS was used to study the resistance to sorghum aphid and maping resistance gene. HN-16 and F1 are resistance to aphid. The genetic analysis with F2 population demonstrated that the microsatellite markers ratio of the resistant family to the susceptible family is 3 ： 1. This indicated that the resistance to sorghum aphid, Melanaphis sacchari, was controlled by a single dominant gene. Microsatellite markers（SSR markers） were used to analyze the F2 bulked segregation population. A molecular marker of SSR linked to the resistance gene was detected which is located on linkage group 9. The linkage map distance was 8. 7 cM.

关 键 词：高粱 抗蚜性 遗传分析 SSR标记 

分 类 号：S514[农业科学—作物学]