红景天水提液对酪氨酸酶抑制效果的初步研究  

Preparatory Study :Effect of Water Extract Solution from Rhodiola sachalinensis A.Boron the Tyrosinase Activity

作　　者：龚静[1] 张飞伟[1] 韩锐[1] 吕雪艳[1] 王丽丽[1] 伊芬芬[1] 乔代蓉[1] 

机构地区：[1]四川大学生命科学学院四川省生物信息及代谢工程共享实验平台,成都610065

出　　处：《四川大学学报（自然科学版）》2006年第2期468-471,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省重点项目(03FG013-015)

摘　　要：To discuss the inhibiting mechanism of water extraxt solution from Rhodiola on theTyrosinase activity.Tyrosinase inhibiting rate is determined and Lineweaver-Burk curve is made by determining the tyrosinase activity before and after drug（water extract solution） interference oxidant of method,on which we deduce the inhibiting types of the drug.The water extract solutioncan inhibit the tyrosinase activity remarkably with the half inhibiting concentration IC50 3.9g/L.The water extract solution can inhibitthe tyrosinase activity and it is thenoncompetitive inhibitor of tyrosinase.To discuss the inhibiting mechanism of water extraxt solution from Rhodiola on theTyrosinase activity. Tyrosinase inhibiting rate is determined and Lineweaver-Burk curve is made by determining the tyrosinase aetivity before and after drug（water extraet solution） interferenee oxidant of method, on whieh we deduee the inhibiting types of the drug. The water extraet solutionean inhibit the tyrosinase activity remarkably with the half inhibiting eoneentration IC50 3.9g/L. The water extraet solution can inhibitthe tyrosinase activity and it is thenoncompetitive inhibitor of tyrosinase.

关 键 词：酪氨酸酶活性 高山红景天 水提液 抑制效果 BERKELEY 药用植物 生长环境 酶抑制剂 抑制作用 高纬度地区 

分 类 号：TQ658.2[化学工程—精细化工] Q949.751.1[生物学—植物学]