高效毛细管电泳分离二肽化合物  

Separation of Dipeptides by High Performance Capillary Electrophoresis

作　　者：程燕[1] 白敏[1] 陈彦[1] 王东芝[1] 石运伟[1] 尤进茂[1] 

机构地区：[1]曲阜师范大学化学科学学院,山东省曲阜市273165

出　　处：《曲阜师范大学学报（自然科学版）》2006年第2期85-88,共4页Journal of Qufu Normal University(Natural Science)

基　　金：国家自然科学基金(20075016)资助项目

摘　　要：以咔唑-9-乙基氯甲酸酯(CEOC)作为柱前衍生试剂,在胶束电动色谱模式下对16种二肽进行了分离,研究了用该试剂衍生的二肽物分离的几个关键的条件,在14 min内实现了16种CEOC二肽衍生物的分离.A new method for the separation of CEOC-dipeptide derivatives under micellar electrokinetic capillary chromatography and using 9-（2-carbazole） ethyl chloroformate （CEOC） as derivatization reagent has been developed. The effect of several key factors such as buffer concentration, SDS concentration, pH value, and organic modifier on the separation are studied. Under the proposed conditions, 16 dipeptide derivatives are resolved with a good baseline resolution.

关 键 词：二肽 胶束电动色谱 衍生化 

分 类 号：O657.8[理学—分析化学]