一株好氧反硝化细菌生理生态特征的研究  

Characteristics of psammophytes of an aerobic denitrifier

作　　者：马放[1] 周丹丹[2] 王宏宇[1] 董双石[1] 

机构地区：[1]哈尔滨工业大学市政环境工程学院,黑龙江哈尔滨150090 [2]中国地质大学水资源与环境学院,北京100081

出　　处：《哈尔滨工业大学学报》2006年第4期575-577,共3页Journal of Harbin Institute of Technology

摘　　要：经测定一株好氧反硝化菌X31菌株的生理生化指标,鉴定该菌株为假单胞菌属（Pseudomonas）,对该菌株进行16S rDNA测序后确定为Pseudomonas chloritidismutans.该菌株在完全好氧的条件下可高效地将NO3^-反硝化为N2,并不产生NO2^-的累积.其培养特征为ORP降低和反硝化产碱.当初始的氧化态氮浓度为150 mg/L左右时,DO值对该菌株的反硝化效果没有影响；而pH值对反硝化效果影响很大,反硝化的最适pH值为中性或偏碱性；反硝化的最适温度为30～35℃.An aerobic denitrifier named X31 was identified to be Pseudomonas through physiology-biochemical test. The result of 16SrDNA sequencing of X31 was Pseudomonas chloritidismutans. The strain had good effect of denitrification in absolute environment （DO 〉 8 mg/L） and no NO2^- accumulation, when respiring with NO3^-. The nitrogen removal rate of the strain was not affected by DO when the concetration of NO2^- - N was about 150 mg/L. The optimum temperature of X31 was 30～35℃, optimum pH was neutral or a little bit alkaline.

关 键 词：PSEUDOMONAS chloritidismutans 生理生态 好氧反硝化 

分 类 号：Q939.9[生物学—微生物学]