反相高效液相色谱法测定氨茶碱人体血药浓度  

RP-HPLC method for the determination of aminophylline in serum

作　　者：杨积平[1] 裕海明[1] 

机构地区：[1]安徽省蚌埠市第三人民医院药剂科,安徽蚌埠233000

出　　处：《安徽医药》2006年第4期263-264,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的 建立HPLC法测定血浆中氨茶碱浓度的方法。方法 采用反相高效液相色谱法测氨茶碱血药浓度。C18柱，流动相为甲醇-水（32：68，v／v），检测波长275nm。结果 在5～25mg·L^-1浓度范围内线性良好，r=0．9997最低检测限为1mg·L^-1，平均回收率为94．12％～108．59％，日内误差与日问误差均小于8％。结论 该法操作简便、快速、准确，可用于氢茶碱的药物监测。Aim To develop RP-HPLC method for the determination of aminophylline in serum. Methods Chromatographic separation has been achieved on C18 column with methanol：water （32： 68,V/V） as the mobile phase. Detection was set at UV 275nm. Result The calibration curve was linear between 5 mg ·L^-1 and 25 mg ·L^-1 with a regression coefficient of 0. 9997. The lower limit of detection of aminophylline in serum was 1 mg ·L^-1. The recovery rate was 94.12% ～ 108.59%. Tnter-day RSD and intra-day RSD of aminophylline were less than 8%. Conclusion The method was simple,rapid and accurate. It could be successfully used for TDM.

关 键 词：高效液相色谱法 氨茶碱 血药浓度 

分 类 号：R96[医药卫生—药理学]