驱蚊香草愈伤组织的诱导和增殖  

Effects on induction and proliferation of Pelargonium graveolens

作　　者：杨春燕[1] 刘树楠[1] 周吉源[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079

出　　处：《华中师范大学学报（自然科学版）》2006年第1期86-89,共4页Journal of Central China Normal University：Natural Sciences

摘　　要：以驱蚊香草(Pelargonium graveolens)的幼嫩叶片为外植体,接种于附加不同浓度的NAA、2,4-D、6-BA、KT及其组合的M S固体培养基上,结果表明:单独使用4种植物生长调节物质对驱蚊香草愈伤组织的诱导在一定浓度范围内都有效果,其中单独使用KT时效果不太明显;最佳的组合培养基为M S+2,4-D 0.2 m g/L+6-BA 0.5 m g/L.在黑暗和光照培养条件下,愈伤组织的增殖呈“S”型,且生长周期均为30 d.Yong leaves of mosquito Pelargonium graveolens which were used as explants, were cultured in medium MS supplement with different kinds,different concentrations and combinations of plant growth regulators. The results showed that：there was no callus when medium MS supplied with only KT. MS＋2,4-D 0.2 mg/L＋6-BA 0. 2 mg/L was the optimum medium for callus induction. When cultured in the dark and the white light, the callus growth curves were in the shape of＂S＂. The cells grew more rapidly in the white light than in the dark.

关 键 词：愈伤组织 诱导 增殖 驱蚊香草 

分 类 号：Q813.1[生物学—生物工程]