体外亚硒酸氢钠致突变和抗突变测试  

MUTAGENICANDANTIMUTAGENICDETECTIONOFSODIUMBISELENITEINVITRO

作　　者：吴金龙 石根勇 陈新霞 王丽云 

机构地区：[1]江苏省卫生防疫站毒理室

出　　处：《癌变．畸变．突变》1996年第2期100-103,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：亚硒酸氢钠（ＮａＨＳｅＯ３）的致突变和抗突变作用尚无定论，本文应用稍加修改的ＳＯＳ／Ｕｍｕ显色试验对其这方面的作用作了研究。结果发现，ＮａＨＳｅＯ１剂量在０．６７－８．００ｍｇ／ｍｌ范围内，无Ｓ９时，ＳＯＳ反应的诱导率（Ｒ）有剂量一效应关系，最低诱导剂量为１．８６ｍｇ／ｍｌ：存在Ｓ９时，各剂量组的Ｒ值均接近于１。结果还揭示ＮａＨｓｅＯ３能明显抑制丝裂霉素Ｃ（ＭＭＣ）诱发的β－半乳糖苷酶（ＢＧａｓｅ）活性，其剂量－效应曲线呈抛物线。据此曲线的方程式，估得２０％、４０％和６０％抑制的剂量分别为０．４８，１．３７和３．２１ｍｇ／ｍｌ；最大有作用剂量为３．５８ｍｇ／ｍｌ，其抑制率为６１％，这些结果表明：ＮａＨＳｅＯ３可能是ＳＯＳ的直接诱导剂，同时又是ＭＭＣ诱发ＳＯＳ反应的强抑制刑，且可在无明显诱导作用的剂量（＜１．８６ｍｇ／ｍｌ）下，即可有抗ＭＭＣ的诱导作用。Mutagenic and antimutagenic effects of NaHSeO3 were investigatcd with Oda'sSOS/Umu chromotest,a few alterations were noticed.The results showed that the inductionratios were dcpend on the dose of NaHSeO3 at range of 0.67- 8.00mg/ml without S9 and theminimum induction dose was 1.86mg/ml,but the induction ratios were all less than 2 atrange of the doses tested with S9.The results also suggested that NaHSeO3 can significantlyinhibit theβ-galactosidase activity induced by mitomycin C(MMC)and the dose-effectcurve was in shape of parabola. From the equation of this curve,the doses of 20%,40%and60%induction of the activity were estimated to be 0. 48,1.37 and 3.21mg/ml NaHSeO3 re-spectively and the most effective dose to be 3.58mg/ml at which NaHSeO3 can inhibit 61%ofthe activity induced by MMC. These results indicated that NaHSeO3 may be a direct inductorof SOS and at the dose(below 1.86mg/ml)without mutagenicity NaHSeO3 can drastically in-hibit the SOS response induced by MMC.

关 键 词：硒化合物 亚硒酸氢钠 SOS反应 丝裂霉素 抗突变 

分 类 号：R994.6[医药卫生—毒理学]