地鼠血清培养异合卵制备人精子染色体的研究  

STUDY OF HUMAN SPERM CHROMOSOME BY CULTURINGHAMSTER OVA WITH HAMSTER SERUM

作　　者：高晓平[1] 李丹[1] 吴无畏[1] 吴德光[1] 

机构地区：[1]四川省计划生育科学研究所

出　　处：《癌变．畸变．突变》1996年第2期117-121,共5页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：本文首次应用地鼠血清培养异合卵制备人精子染色体标本，获得显著的效果。同时应用胎牛血精进行了比较，在两种血清中精子染色体形成率分别为１０２．４５％和５８．２８％，可供分析核型获得率分别为７６．１５％和３１．５１％，两组间存在非常显著的差异（Ｐ＜０．００１）．观察分析了６例供精者的４９８个精子核型，数目异常和结构异常发生率分别为０．８％和２．８％。本研究提示地鼠血清培养同种属卵，可促进卵细胞在体外的进一步成熟，并有助于人精原核的发育及其染色体的形成。This study reporr a method for preparating human sperm chromosome with ham-ster serum for postinsemination culture of the zona-free hamster ova.The rate of analyzablekaryotype was markedly increased.Comparison hamster serum with fetal bovine serum.Theaverage rate to reach the first-cleavage was 102.45%in the inseminated oocytes versus 58.28%.The rate of observable karyotyping in two sera were 76.15%versus 31.51%(P<0.001).Spontaneous incidence of sperm chromosome aberrations was analyzed in a total of 498spermatozoa obtained from six donors.Incidence of aneuploidy and structural abnormalitywere 0.8%(hypohaploidy)and 2.81%,respectively.This result suggest that the hamsterserum may be more suitable to the further maturity of hamster ova in vitro and contribute tothe growth and development of male pronuclear chromosome.

关 键 词：金黄地鼠血清 异合卵培养 人精子染色体 

分 类 号：R321－33[医药卫生—人体解剖和组织胚胎学]