衍生化气相色谱法检测瘤胃液和青贮液中乳酸对映体和挥发酸  

Gas chromatography assay of lactic acid isomers and VFA in rumen and silage fluids by pre-column chiral derivation procedure

作　　者：常影[1] 邱静[2] 魏吉安[1] 孟庆翔[1] 

机构地区：[1]动物营养学国家重点实验室中国农业大学 [2]中国农业大学理学院,北京100094

出　　处：《中国农业大学学报》2006年第2期49-53,共5页Journal of China Agricultural University

基　　金：国家自然科学基金资助项目(30270944)

摘　　要：利用手性衍生化试剂L-薄荷醇和气相色谱柱前衍生化，将瘤胃液和青贮液中L-和D-乳酸对映体转化为酯类非对映体对。建立同时分析发酵液中乳酸对映体和挥发酸的气相色谱检测方法。样品乳酸对映体的质量浓度在0．05～10．00mg／mL范围内时．标准曲线的决定系数分别达到R^2=0．9995和R^2=0．9996。乳酸对映体的回收率为96．15％～108．24％．且相对标准偏差小于5％。采用本方法检测瘤胃液和青贮液样品的L-和D-乳酸对映体含量，并且与酶学检测法进行比较，2种方法的测定结果没有显著性差异（P〉0．05）。发酵液中挥发酸经衍生化处理。可以与L-和D-乳酸对映体在同一色谱条件下检测，各组分在0．05～5．00mg／mL范围内线性良好，R^2=0．9982～0．9994。乳酸对映体和挥发酸的色谱分析在6min完成。A novel procedure for determination of lactic acid isomers in the rumen and silage fluid was developed based on pre-column chiral derivation with L-menthol. The determination showed a linear response within the concentration range of 0.05 and 10.00 mg/mL for L- and D-lactate isomers with the correlation coefficients of 0.999 5 and 0.999 6, respectively. The recovery of each isomer averaged between 96.15 % and 108.24% （RSD 〈 5 % ）. A comparison of analytical results of lactate isomers on the rumen and silage fluid established between the enzyme procedure and that in this studyshowed no significant difference （P 〉0.05）. Using gas chromatography conditions described here, 6 volatile fatty acids （VFA） including acetate, propionate, iso-butyrate, butyrate, valerate and iso-valerate acids were simultaneously analyzed with both lactate isomers, having high correlation coefficients of 0.998 2 and 0.999 4 within the VFA concentration range from 0.05 to 5.00 mg/mL. Fermentation fluids were run for 6 min for both the isomers of lactic acid and VFA assay.

关 键 词：乳酸对映体 气相色谱法 瘤胃液 青贮液 

分 类 号：S816.17[农业科学—饲料科学]