籽粒含油量的选择轮次对BHO群体高油玉米的化学成分、活体外消化率和发酵参数的影响  

Influence of oil selection cycles of BHO population on chemical compositions,in vitro ruminal digestion characteristics and fermentation traits of HOC grain

作　　者：常影[1] 陈绍江[2] 孟庆翔[1] 

机构地区：[1]动物营养学国家重点实验室中国农业大学 [2]中国农业大学农学与生物技术学院,北京100094

出　　处：《中国农业大学学报》2006年第2期54-60,共7页Journal of China Agricultural University

基　　金：国家自然科学基金资助项目(30070562)

摘　　要：在北农大高油(BHO)玉米群体的16个选择轮次中隔轮选取籽粒样品为试验材料,利用活体外人工瘤胃产气量方法,研究HOC籽粒含油量的选择进程对BHO群体籽粒的化学组成、活体外干物质和淀粉消化率以及瘤胃发酵参数的影响。BHO群体的籽粒含油量从第2轮的6.84%提高至第16轮的13.20%,分别与籽粒淀粉和蛋白质含量呈现显著的负相关(r=-0.84,P<0.001)和正相关(r=0.74,P<0.001)。随着含油量选择进程的提高,HOC籽粒的活体外产气速度和淀粉消化率线性增加(P<0.001),这可能源于BHO群体的淀粉结构随含油量选择进程发生了改变。理论最大产气量和活体外干物质消化率随含油量选择轮次的提高而线性降低(P<0.001)。活体外发酵6 h的pH、总挥发酸(mmol/L)和丙酸比例(%)线性提高(P<0.001),乙酸和丁酸的比例线性下降(P<0.001)。活体外发酵24 h以后,不同选择轮次间的各项发酵参数均无显著差异。An in vitro gas production experiment was conducted to investigate the effect of oil selection progress of Beinongda high oil （BHO） population on chemical composition, ruminal digestion characteristics and fermentation traits in vitro. The experimental HOC grains were alternately obtained from 16 oil-selecting cycles of BHO population. With genetic selection progress, the fat content of BHO population linearly increased from 6.84% in cycle 2 to 13.20% in cycle 16. The starch and protein content in HOC grain quadratically responded to the selection progress, and there were close correlations between the content of starch and fat （r = - 0.83, P 〈 0.001 ） in one hand and between the content of protein and fat （r = 0.75, P〈0.001） in the other hand. Rate and extent of starch digestion significantly increased in a linear fashion with increased selecting cycles, indicating that genetic selection for oil in such BHO population probably altered the structure of endosperm layers or texture of starch granules. Accumulative gas amount during 48 h fermentation and DM digestibility at 24 h incubation time significantly declined with increased selecting cycles. This may be attributable to the reduced starch content with the increased fat content, rather than to any direct effect of increased fat. As fat content increased, the pH value, total VFA concentration and propionate molar percentage also significantly increased in a linear fashion and molar Droportions of acetate and butyrate linearly declined at 6h incubation time. However, these values did not differ at 24 h incubation time.

关 键 词：高油玉米籽粒 选择轮次 活体外瘤胃消化率 活体外瘤胃发酵参数 

分 类 号：S816.2[农业科学—饲料科学] S5-033[农业科学—畜牧学]