Toll样受体基因表达水平在儿童时期的变化及其意义  

Expression of Toll-like receptors genes in mononuclear cells during childhood

作　　者：陶月红[1] 王莹[1] 朱晓华[1] 王卫平[1] 杨毅[1] 

机构地区：[1]复旦大学附属儿科医院,上海200032

出　　处：《中国儿童保健杂志》2006年第2期160-162,共3页Chinese Journal of Child Health Care

基　　金：国家自然科学基金(30371210)

摘　　要：【目的】观测单个核细胞Toll样受体（Toll—likereceptor．TLR）2、3、4的基因表达水平在儿童时期的变化．探讨其在天然免疫发育中的意义。【方法】收集0～15岁健康儿童的外周血。共259份标本，分离单个核细胞，采用RT-荧光定量PCR法测定TLR2、3、4mRNA。【结果】婴幼儿TLRs基因表达水平较高，其后有所下降，男童至青春期达到第二个高峰。性别对TLRs mRNA水平具有影响，表现为学龄前期儿童TLR2、3、4mRNA水平女童高于男童，而9岁以后T1、R2、3、4mRNA水平女童普遍低于男童。【结论】TLRs基因表达水平存在年龄和性别相关的变化。年幼儿TLRs的高表达表明在婴幼儿时期·天然免疫系统对外界病原体的识别就已经相当完善。学龄前期及青春期前后儿童．确实存在性别有关的TLRs的表达差异。可能与其性激素的水平有关。[Objectives] To observe the age-related changes of genes expression for Toll-like reeeptors（TLR） 2, 3, 4 in mononuclear cells during childhood, and to probe into their roles they play in innate immunity development, [Methotis] Total 259 blood samples were collected from health children age 〈15 years old. Mononuclear cells were separated and total RNA was isolated with Trizol reagent. By using RT-quantitative PCR, the mRNA levels of TLR2, 3, 4 were detected. [Results] The gene express!ons for TLR2, 3, 4 were in high levels in children age 〈3 years, and then were declined. The mRNA levels of TLRs reached the second peak were in adolescence. Gender could influence the gene expressions for TLRs, shown as, in preschool children, TLR 2, 3, 4 mRNA levels were higher in girls than those in boys, however, after age 9, TLRs mRNA levels were general lower in girls than those in boys. [Conclusions] The age and gender - related changes of gene expressions for TLR2, 3, 4 do exist in mononuclear cells during childhood. The high mRNA levels of TLRs showed even in infants, and it was indicated that the innate immune system of children has the perfect power to recognize foreign pathogens. Near adolescence, gender-related varieties of TLRs may induced by sexual hormone.

关 键 词：单个核细胞 TOLL样受体 基因表达 儿童 免疫 

分 类 号：R722.1[医药卫生—儿科]