一株提高原油采收率嗜热菌株的性能评价  

Performance evaluation of a thermophilic MEOR strain.

作　　者：张瀚奭 段传慧[2] 高光军[2] 程海鹰[1] 王静[2] 张前前[1] 

机构地区：[1]中国海洋大学海洋化学化工学院,山东青岛266003 [2]胜利油田有限公司采油工艺研究院,山东东营257000

出　　处：《石油钻采工艺》2006年第2期46-48,51,共4页Oil Drilling & Production Technology

基　　金：国家"十五"攻关项目"极端微生物石油开采技术研究(2003BA613A-08)"的部分内容

摘　　要：从胜利油田产出液中分离得到一株嗜热菌NA-3。经鉴定为芽胞杆菌属。该菌为[艹卓]兰氏阳性，杆状，兼性厌氧，有运动性，茼体大小为（0．5～0．8）μm×（1．4～2．5）μm。最适生长温度为70℃，最适生长pH为7．0～8．0，耐受矿化度可达10g／L。能产酸和生物表面活性剂。可使培养液的表面张力从72mN／m降至34mN／m。pH值从7．0降至5．2。试验表明，NA-3菌液在物理模拟条件下经多轮次驱替仍能提高采收率，微生物提高采收率累计可达11～15个百分点。在微生物采油方面将有很好应用前景。A thermophilic bacterium of NA-3 was isolated from produced fluid in Shengli oilfield,and identified that it was a member of Bacillus sp.The NA-3 is Gram-positive,rod-shaped in(0.5-(0.8))μm×((1.4)-2.5)μm,facultative anaerobe and motive.The optimum growth temperature is 70 ℃,pH is 7.0.salinity resist is 10 g/L.NA-3 could reduce nutritive fluid surface tension from 72 mN/m to 34 mN/m,pH from(7.0) to 5.2.Experiment shows that NA-3 can enhance the oil recovery to 11-15 percent,and it has great potential application in microbial enhanced oil recovery.

关 键 词：微生物采油 嗜热 兼性厌氧菌 性能评价 室内试验 

分 类 号：TE357.9[石油与天然气工程—油气田开发工程]