椰心叶甲ITS、5.8S rDNA序列分析  

Analysis of the sequencing of internal transcribed spacer and 5.8S ribosomal DNA from Brontispa longissima

作　　者：黄华平[1] 杨腊英[1] 程子路[1] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所热带作物生物技术国家重点实验室,海南儋州571101

出　　处：《广东农业科学》2006年第4期56-59,共4页Guangdong Agricultural Sciences

基　　金：科技基础性工作和社会公益研究专项(2004DIB3J073)

摘　　要：利用PCR和DNA测序技术,克隆了椰心叶甲的内转录间隔区ITS及5.8S rDNA序列,其全长为2 107 bp,并对这一序列进行了详细的分析,为研究椰心叶甲地理种群的差异及其不同类群之间的遗传关系,并重构椰心叶甲从原产地到各受灾地区的传播路线等方面奠定了分子基础,对制定椰心叶甲的综合防治策略具有指导意义。Brontispa longissima Brontispa longissima. Internal transcribed spacer and of Brontispa longissima （Gestm） were amplified by tyolymerase chain reaction and sequenced after the amplified gene segment was cloned. It＇s longest ,sequence was 2 107 bp. The sequenees were aligned and analyzed by GeneBank and DNAStar 5.0 software. It can research difference in geographical populations, genetic relation of populations and invasion route. It is important that guiding significance to building integrate control strategy.

关 键 词：椰心叶甲 RDNA ITS1 ITS2 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]