用补体裂解片断C4d监测大鼠移植肾慢性排斥反应  

C4d: an effective indicator for monitoring chronic rejection of renal allograft in rat

作　　者：祝清国[1] 赵亚昆[1] 李井泉[1] 于泳[1] 韩志军[1] 仇宇[1] 董志[2] 

机构地区：[1]哈尔滨医科大学附属第二医院泌尿外科,150086 [2]黑龙江省鸡西矿业集团杏花矿医院外科

出　　处：《中华器官移植杂志》2006年第4期225-227,共3页Chinese Journal of Organ Transplantation

基　　金：哈尔滨市科技攻关计划资助项目(2005AA9CS1168)

摘　　要：目的探讨补体裂解片断C4d在监测大鼠肾移植后慢性排斥反应中的作用与意义。方法以封闭群Wistar大鼠为供者,SD大鼠为受者,建立大鼠肾移植模型。将42只受者随机平均分为2组,A组在肾移植术后10d内给予小剂量环孢素A(5mg·kg-1·d-1)治疗;B组除使用环孢素A外,另给予霉酚酸酯10mg·kg-1·d-1灌胃。术后第3、5和10周观察各组移植肾形态变化及移植肾组织中C4d沉积情况。结果从术后第3周起,A组开始出现轻微慢性排斥反应的病理改变,并随着时间的推移逐渐明显。B组于术后第5周开始出现慢性排斥反应的病理改变,程度轻于A组。术后第3周A组即出现明显C4d沉积,至第10周时沉积已较广泛。B组也有C4d沉积,但沉积程度较A组同时段轻(P<0.05)。结论移植肾组织中C4d的沉积早于慢性排斥反应的病理改变,可作为预测慢性排斥反应的有效指标。Objective To explore the significance of complement split product C4d in monitoring chronic rejection of renal allografts in rats. Methods Healthy closed population rats were used as do nors and SD rats as recipients. The Wistar to SD model of graft rejection was developed. All the 42 recipients were randomly divided into 2 groups： group A receiving nothing except CsA （5mg· kg^-1 · d^-1 ） in the first 10 days after operation, and group B receiving MMF （10 mg/kg） and CsA after opera tion. On the 3rd, 5th and 10th week, all the allografts were tested by light microscopy and immunofluorescence. Pathological changes of the kidneys and the expression of C4d in allograft tissue were ob served. Results From the 3rd week, the rats in group A showed light pathological changes of chronic rejection and they became more and more obvious as time increased. Pathological changes occurred in group B at the 5th week and lighter than in group A. At the same time, CAd deposition in PTC was obviously observed on the 3rd week in group A, and on the 10th week C4d widespread deposition in al lografts. Conclusion The deposition of complement split product C4d in allografts appears earlier than pathological change of chronic rejection, which can be regarded as a significant indicator to predict chronic rejection of renal allografts.

关 键 词：补体4 肾移植 移植物排斥 大鼠 

分 类 号：R699.2[医药卫生—泌尿科学]