锰对大豆POD和EST同工酶谱的影响  

Manganese Effects on POD and EST Isozyme Patterns of Soybean

作　　者：徐根娣[1] 孙和和[1] 刘鹏[1] 包海英[1] 

机构地区：[1]浙江师范大学植物学重点实验室,浙江金华321004

出　　处：《河南农业科学》2006年第4期46-50,共5页Journal of Henan Agricultural Sciences

基　　金：浙江省自然科学基金(405135)资助;金华市科技项目

摘　　要：采用垂直平板聚丙烯酰胺凝胶电泳,研究了2个大豆品种(华春18和浙春3号)在不同时期、不同锰浓度处理下的过氧化物(POD)同工酶和酯酶(EST)同工酶的变化情况。结果表明,随锰浓度的增加,华春18的POD同工酶的酶带数下降,EST同工酶的酶带数变化不大,浙春3号的2种同工酶的酶带数变化不大;2个大豆品种在不同时期的POD和EST同工酶的酶带数也变化不一,这与各品种对锰胁迫的抗性有关。The isozymes of POD and EST in two varieties of soybean（Huachun No. 18 and Zhechun No. 3） under the different concentration stress of manganese at two different growth stages（3 - trifoliate and 5 - trifoliate） were studied with vertical polyacrylamide gel electrophoresis. The results showed that the POD isozyme quantity decreased, while the EST isozyme quantity did not changed obviously when the manganese concentration increased for Huachun No. 18, but for Zhechun No. 3, the isozyme quantities both of POD and EST had no evident changes. There were differences in the isozyrne quantities of POD and EST of the two varieties at different growth stages, and this difference was related to the variety resistance against manganese stress.

关 键 词：锰 胁迫 过氧化物酶 酯酶 同工酶 大豆 

分 类 号：S565.1[农业科学—作物学]