硒和镉联合作用对大鼠肝脏TERT mRNA表达的影响  

Effects of selenium and cadmium on TERT expression in rat liver

作　　者：陈华洁[1] 余日安[1] 贺凌飞[2] 夏涛[1] 吴志刚[1] 杨克敌[1] 陈学敏[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系教育部环境与健康重点实验室,湖北武汉430030 [2]武汉大学中南医院

出　　处：《毒理学杂志》2006年第2期71-74,共4页Journal of Toxicology

基　　金：国家自然科学基金资助项目(30271110)

摘　　要：目的 研究亚硒酸钠、氯化镉及其联合作用对大鼠肝脏端粒酶逆转录酶（TERT）mRNA表达的影响。方法 健康雄性SD大鼠随机分组，每组动物5只。对照组，硒组（2．5、5和10μmol／kg Na2SeO2）、镉组（5、10和20μmol／kgCdCl2）以及硒镉联合作用组。染毒48h后取出肝脏，用RT-PCR方法检测TERT基因的表达。结果 与对照组比较，3个剂量硒组的TERT mRNA虽有增高，但P〉0．05，而3个剂量的镉组TERT mRNA表达均增多（P〈0．01）；在硒镉联合作用组中，虽然3个剂量的硒均可分别使3个剂量的镉诱导的大鼠肝脏TERT mRNA表达下降，但仅在2．5μmol／kg Na2SeO3＋5μmol／kgCdCl2组、2．5μmol／kg Na2SeO3＋10μmol／kg CdCl2组和5μmol/kg Na2SeO3＋10μmol／kg CdCl2组，与相应剂量的镉组比较，TERT mRNA水平降低，差异具有显著性。10μmol／kg Na2SeO3＋5、10和20μmol/kg CdCl2联合作用组与对照组相比，TERT mRNA水平均升高，与对应镉组相比，差异无显著性。结论 镉在5～20μmol／kg的剂量条件下可以诱导大鼠肝脏高表达TERT mRNA，而一定剂量的硒对一定剂量镉引起的TERT mRNA的表达具有一定的拮抗作用。Objective To study the effects of sodium selenite（ Na2 SeO3 ）, cadmium chloride（ CdCl2 ） and their joint action on expression of telomerase reverse transcriptase（TERT） mRNA in rat liver. Methods Male SD rats were divided randomly to 16 groups, each group had 5 animals. The groups include ： control group, Se groups （ Na2 SeO at the doses of 2.5,5 and 10 μmol/kg, Cd groups （CdCI： at the doses of 5,10 and 20 μmol/kg, Se（ Na2 SeO3 at the doses of 2.5,5 and 10 μmol/kg ＋ Cd（ CdCl2 at the doses of 5,10 and 20 μmol/kg groups. After 48 h of the first injection ,the expression of TERT mRNA was measured with Reverse transcription polymerase chain reaction （RT-PCR）. Results Although both Se at the doses of 2.5,5 and 10 μmol/kg and Cd at the doses of 5,10 and 20 μmol/kg could increase rTERT expression in rat liver,compared with control group, statistical analysis yielded P 〈 0.01 only in Cd groups. In the joint action groups of Se and Cd, Se at the doses of 2.5,5 and 10 μmol/kg could decrease rTERT over expressions induced by Cd at the doses of 5,10 and 20 μmol/kg respectively, but statistical analysis showed that rTERT expressions in 2.5 μmol/kg Na2 SeO3 ＋ 5 μmol/ kg CdCl2 group,2.5 μmol/kgNa2SeO3 ＋ 10 μmol/kgCdCl2 group and 5 μmol/kgNa2SeO3 ＋ 10 μmol/kgCdCl2 group were lower than those of correspondent Cd group respectively and significantly. The expression of rTERT mRNA in 10 μmol/kgNa2 SeO3 ＋ 5,10 and 20 μmol/kgCdCl2 groups were no significant different from correspondent Cd groups, but higher than that of control significantly. Conclusion It was suggested that cadmium at the doses of 5 μmol/kg～ 90 μmol/kgcan induce overexpression of TERT mRNA in rat liver. Selenium at the doses of 2.5 ～ 5 μmol/kg have antagonistic effect on overexpression of TERT induced by Cd in rat liver.

关 键 词：硒 镉 端粒酶逆转录酶 

分 类 号：R114[医药卫生—卫生毒理学]