不同频率电针对大鼠杏仁核内生长抑素mRNA表达的影响  

Effect of Different Frequencies of Electroacupuncture on Somatostatin mRNA Expression in Rats' Amygdaloid Nucleus

作　　者：赵健[1] 熊克仁[1] 李怀斌[1] 

机构地区：[1]皖南医学院人体解剖学教研室,芜湖241001

出　　处：《针刺研究》2006年第2期103-106,共4页Acupuncture Research

基　　金：安徽省自然科学基金资助项目(No.010431002)

摘　　要：目的:观察不同频率电针大鼠“足三里”穴对杏仁核内生长抑素(SOM)mRNA表达的影响。方法:雄性SD大鼠30只,随机分为对照组2、Hz电针组和128 Hz电针组,每组10只。采用原位分子杂交方法,观察不同频率电针大鼠一侧“足三里”穴30 min后,大鼠杏仁核内SOM mRNA的表达。结果:电针对大鼠杏仁核内SOM mRNA的表达有上调作用,并且高频电针作用强于低频电针。电针后杏仁核各亚细胞群内SOM mRNA阳性神经元的数量增加以中、小型细胞较为明显;在细胞染色程度上以中等程度染色和淡染细胞的数量增加为主,细胞平均灰度值有所增加。结论:不同频率电针对大鼠杏仁核内SOM mRNA的表达有明显影响。Objective：To observe the effect of different frequencies of electroacupuncture （EA） of ＂Zusanli＂ （ST 36） on the expression of somatostatin （SOM） mRNA in rats＇ amygdaloid nucleus. Methods： Thirty SD rats were randomized into control, 2 Hz-EA and 128 Hz-EA groups with 10 cases in each group. Left ＂Zusanli＂ （ST 36） was punctured and stimulated electrically for 30 min by setting the parameters being 2 Hz and 128 Hz in frequency, 3 V in strength, and 1 ms in the duration of waves. After EA, the rats were transcardically perfused with 1% paraformaldehyde and 1.25% glutaraldehyde and 0.1 mol/L, phosphate buffer solution. The brain tissues were sampled to cut into sections （40 μm）. The expression of SOM rnRNA of amygdaloid nucleus was detected by using in situ hybridization technique. Results： Compared with control group, the number of SOM rnRNA expression positive neurons and the grey-density values of SOM mRNA expression in amygdaloid nucleus on the bilateral sides increased significantly in 2 Hz and 128 Hz EA groups （P〈0.05, 0.01）. There were significant differences between two EA groups in the number of SOM mRNA expression positive neurons and the grey-density values. Conclusion： Both 2 Hz and 128 Hz EA of ＂Zusanli＂ can up-regulate the expression of SOM mRNA in the rat＇s amygdaloid nucleus. The high frequency of EA has a more obvious effect.

关 键 词：生长抑素 MRNA表达 杏仁核 不同频率电针 

分 类 号：R245.97[医药卫生—针灸推拿学]