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作 者:WANG Aihua WANG Jicheng DUAN Delin 王爱华;王继成;段德麟(Institute of Oceanology,The Chinese Academy of Sciences Qingdao 266071,China;The Graduate School of the Chinese Academy of Sciences,Beijing 100039,China)
机构地区:[1]Institute of Oceanology,The Chinese Academy of Sciences Qingdao 266071,China [2]The Graduate School of the Chinese Academy of Sciences,Beijing 100039,China
出 处:《Chinese Journal of Oceanology and Limnology》2006年第2期129-133,共5页中国海洋湖沼学报(英文版)
基 金:Supported by NSFC(No.40376049);Shandong Agricultural Seed Stock Breeding Project.
摘 要:Chondrus is an economically important red algae widely used for food and biochemical purpose.It early development is crucial for the culture and seedling propagation.We chose tetraspores and carpospores of Chondrus ocellatus as examples for experiment of the culture,induction and release in laboratory condition,aiming to understand early development of C.ocellatus and to apply in seedling production.Mature C.ocellatus were collected in Qingdao,China,from Nov.to Dec.2004.After the gametophyte and tetrasporophyte were brushed and washed with sterilized seawater,the algal materials were treated in 1.5%KI for 20 min,then were dried for lh to stimulate the releasing of spores.After the spores released overnight,it were cultured in PES medium,incubated at 18℃,10±2μmol/(m^(2).s^(1))in 12:12h(light:dark).The observation and recording under microscope were carried out.Continuous observation of the early development showed that both tetraspore and carpospore are similar to each other.In general,three stages of the early development were shown being division,discoid crust and seedling stages.To the division stage,the most obvious feature was the increasing of cell number;during the discoid crust stage,the discoid crust had a three-dimensional axis,and it began to differentiate into two types of cells:the basal cells and the apical cells;and to the seedling stage,several protuberances-like appeared on the discoid crusts and formed juvenile seedlings.Carpospores and tetraspores exhibited a similar development process that included division stage,discoid crust stage and seedling stage.
关 键 词:Chondrus ocellatus early development carpospore TETRASPORE discoid crust SEEDLINGS
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