CsA对移植肾受者淋巴细胞免疫相关基因的调控  

The Effect of CsA on Immunological Gene Expression in Renal Transplant Recipients

作　　者：文进[1] 张玉海[2] 田野[2] 贾宝祥[2] 尔秀江[2] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院泌尿外科,北京100730 [2]首都医科大学附属北京友谊医院泌尿外科,北京100050

出　　处：《江西医学院学报》2006年第2期81-84,共4页Acta Academiae Medicinae Jiangxi

摘　　要：目的研究环孢素A(CsA)作用后,移植肾受者淋巴细胞免疫相关基因表达的变化。方法分离移植肾受者在药物作用前后24 h外周血淋巴细胞,将它们总RNA分别逆转录并同基因芯片杂交,通过生物信息学方法比较分析。结果CsA作用24 h后,7个基因显著下调。结论CsA通过以上一系列靶基因发挥药理作用,这为临床治疗提供了新的依据。Objective To scan and analyze the immunological genes of lymphacytes associated with dominant regulation in renal transplant recipients treated with cyclosporine（CsA）. Methods The peripheral blood lymphacytes just before and 24 hours after CsA treatment were isolated. The total RNA of them were reverse-transcripted respectively and hybridized with gene chips. The results were analyzed by bioinformatics. Results In 24 hours after treatment with CsA 7 genes were obviously downregulated. Conclusion A series of target genes mentioned above may be associated with CsA , which provides a new scientific knowledge to clinical treatments.

关 键 词：基因表达调控 肾移植 基因芯片 环孢素A 

分 类 号：R699.2[医药卫生—泌尿科学] R979.5[医药卫生—外科学]