针对HBVS基因的反义锁核酸抗乙肝病毒表达的初探  

Initial study on inhibition of HBV expression by Locked nucleic acid directed at HBV S gene

作　　者：唐盈[1] 王燕菲[1] 

机构地区：[1]广州医学院生物化学教研室,广州510182

出　　处：《江西医药》2006年第4期205-208,共4页Jiangxi Medical Journal

基　　金：广州市科技计划项目(编号:2002Z-108-02)

摘　　要：目的探讨针对HBVS基因翻译起始区的反义锁核酸(LNA)片断体外抗乙型肝炎病毒表达的作用。方法合成三段均互补于HBVS区同一位点的反义核酸片断:锁核酸、反义寡核苷酸、全硫代反义寡核苷酸及无关对照序列,作用于HepG22.2.15细胞,采用ELISA法动态检测细胞上清中HBsAg含量的变化并比较其抗HBV抗原表达作用,以四甲基偶氮唑兰(MTT)法检测LNA对细胞的毒性。结果三段反义寡核苷酸均能抑制HBsAg的表达,作用7d后抑制率分别为52%、42%、45%,其中LNA抗病毒活性最强且对细胞代谢无影响,无关序列无明显抑制作用。结论针对HBVS区的锁核酸体外能有效抑制乙型肝炎病毒的表达,为乙肝的分子治疗开辟了新的前景。Objective To study on inhibition of HBV expression by Locked nucleic acid directed at HBV S gene. Methods Three antisense phosphorothioate oligodeoxy nucleotides （ASON）complementary to the initiator of HBV S gent, were synthesized and assayed for their anti-HBV activity on HepG2 2.2.15 cells with ELISA detection. LNA＇s toxicity on cell was detected by MTr method. Results These ASONs could inhibit the expression of HBsAg with the inhibition rates of 52%,42% and 45% respectively after 7 days. LNA was more effective on anti-HBV than other ASONs and had no effect on 2.2.15 metabolism. Noncomplementary sequence had no obvious inhibition effect. Conclusion LNA has a strong inhibitition effect on HBV,it has a therapeutic potential in the treatment of patients infected with HBV.

关 键 词：肝炎病毒 乙型 锁核酸 寡核苷酸类 反义 2.2.15细胞 

分 类 号：R373.21[医药卫生—病原生物学]