人类重组酸性成纤维细胞生长因子对细胞增殖和凋亡的影响  

Effects of human recombinant acidic fibroblast growth factor on proliferation as well as apoptosis

作　　者：吕文天[1] 赵红光[1] 龚平生[2] 王志成[1] 姜晓燕[1] 刘淑春[1] 李校 龚守良[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021 [2]温州医学院生物与天然药物研究院,浙江温州325035 [3]吉林大学分子酶学工程教育部重点实验室,吉林长春130023

出　　处：《中国实验诊断学》2006年第4期336-340,共5页Chinese Journal of Laboratory Diagnosis

基　　金：国家863专题项目(2002AA2Z3318)

摘　　要：目的通过对人类重组型和野生型酸性成纤维细胞生长因子(raFGF,waFGF)的比较研究,探讨raFGF与促分裂性有关的生物学作用。方法以NIH3T3细胞株,采用MTT法通过细胞增殖实验检测waFGF和raFGF的促分裂性,通过流式细胞术检测细胞凋亡、细胞膜通透性和线粒体膜电位;同时,检测肝素(HS)对aFGF的上述作用的影响。结果raFGF对细胞增殖作用明显低于waFGF,对细胞凋亡的抑制作用及其对细胞膜通透性的增强作用也明显低于waFGF,对线粒体膜电位的增强作用明显低于waFGF。HS使aFGF的上述作用增强。结论raFGF的促分裂作用低于waFGF,而对细胞凋亡的影响不明显;HS对aFGF促分裂作用具有促进效应。Objective AIM To evaluate the biological effect of raFGF concerned with mitogenic acticity through studying the differences between wild - type acidic fibroblast growth factor （ waFGF ） and recombinant aFGF （ raFGF ） . MethodsNIH3T3 cell proliferation was determined with MTT method for study on mitogenie activity. Apoptosis,cell membrane permeability and mitochondria membrane potential were measured with flow cytometry. The effect of heparin sulfate （HS） on aFGF mitogenic acticity was studied at the same time. Results The enhancement of raFGF on cell proliferation was significantly lower than that of waFGF. The effects of raFGF on inhibition of apoptosis and the enhancement of cell membrane permeability all were significantly lower than those of waFGF. The enhancement of raFGF on mitochondria membrane potential was significantly lower than that of waFGF. The HS enhanced the above-mentioned effect of aFGF. Conclusion The mitogenic activity of raFGF is lower than that of waFGF,and raFGF has little effect on apoptosis. The effect of HS on mitogenic activity of aFGF has the enhancement property.

关 键 词：酸性成纤维细胞生长因子 硫酸乙酰肝素 细胞凋亡 细胞膜通透性 线粒体膜电位 

分 类 号：R341[医药卫生—基础医学]