NOS抑制剂对创伤性休克作用的实验研究  

The experimental research of NOS inhibitor effect on the survival rates of rats with traumatic shock

作　　者：于亚新[1] 谷贵山 祁柏宇[2] 欧海英[1] 徐晶华[2] 郭鼎[2] 

机构地区：[1]吉林大学第一医院,吉林长春130021 [2]吉林大学基础医学院法医学教研室

出　　处：《中国实验诊断学》2006年第4期355-357,共3页Chinese Journal of Laboratory Diagnosis

基　　金：霍英东教育基金(81039)

摘　　要：目的探讨NOS抑制剂对抢救创伤性休克的效果和影响。方法通过大鼠创伤性休克模型,用NOS检测试剂盒检测失血后各时间段大鼠心、肝、脾、肺和肾脏中iNOS和cNOS的改变。记录下给予NOS抑制剂L-精氨酸(L-Arg)和氨基胍(AG)后各组大鼠的存活率和死亡率。结果对照组在休克时检测出cNOS,创伤休克组0.5 h检测cNOS值高于对照组,而iNOS值变化不明显,创伤休克2.5 h,所有脏器cNOS值开始缓慢有规律降低,反而测得的iNOS值开始有规律的增高,创伤休克5 h脏器中cNOS有规律降低,而iNOS有规律升高。常见的几种NOS抑制剂(L-Arg和AG)与对照组比较存活率有明显提高(P<0.01)。结论NOS抑制剂抢救创伤性休克,可明显提高大鼠24 h存活率。Objective To evaluate the effect of NOS inhibitor on the survival rates of rats with traumatic shock.Methods Rat models of traumatic shock were established in SD rats. The constitutive NOS and inducible NOS in the heart, liver, spleen and kidney were measured 0.5,2.5,5 h after traumatic shock,and the survival rates and death rates of the rats who received NOS inhibitorsd were recorded.Results cNOS are detected in control group during shock, while eNOS were higher in. traumatic shock group than in control one 0.5 h after traumatic shock but there is no change in iNOS between the two groups. 2.5 h after shock, eNOS began to decrease slowly and regularly in the organs, while iNOS began to increase regularly. 5h after shock,eNOS decreased regularly while iN OS increased regularly. Compared with control group, the survival rates of L- Arg group and AG group improved significantly （P 〈 0.01 ）. Conclusion N OS inhibitor can significantly increase the survival rate at 24 h of the rats with traumatic shock .

关 键 词：NOS抑制剂 创伤性休克 存活率 

分 类 号：R641[医药卫生—外科学]