万古霉素耐药肠球菌的检测和分子流行病学分析  

Detection and analysis of molecular epidemiology in vancomycin-resistant enterococci

作　　者：李爽[1] 张正[1] 

机构地区：[1]北京大学人民医院检验科,北京100044

出　　处：《中国实验诊断学》2006年第4期372-375,共4页Chinese Journal of Laboratory Diagnosis

基　　金：北京市自然科学基金项目(No.7012013)

摘　　要：目的为了解本院万古霉素耐药肠球菌(VRE)的流行情况,对VRE株进行实验室检测和分子流行病学检测分析。方法采用多重PCR及测序检测万古霉素耐药基因van,Etest法测定VRE株对万古霉素、替考拉宁的耐药表型;应用脉冲场凝胶电泳(PFGE)对11株VRE进行检测流行病学分析。结果检出1株VanA、4株VanB、5株VanC1,1株VanC2,对万古霉素、替考拉宁的药敏表型与基因型一致;11株VRE中2号vanB株与5号vanB株显示出相同片段,其他VRE株则分别有多于5个区带不同。结论实验室准确检测VRE对防止VRE感染和流行是非常重要的;PFGE电泳分析结果表明了本院11株VRE医院感染为散发。Objectlve To study the prevalence of vaneomycin-resistant enterococci （VRE） in hospital we detected and analyzed the molecular epidemiology of 11 VRE.Methods van gene were detected with multiplex PCR and DNA sequencing.Pulsed field gel electrophoresis（PFGE） was used to analyze the similarities of 11 VRE. Results 1 VanA, 4 VanB, 5 VanC1, 1 VanC2 were detected and the phenotypes confirm to the genotypes; No.2 and No,5 enterococcus showed the same DNA bands.Others showed more than 5 different DNA bands.Conclusion It is important for clinical laboratory to detect VRE with right methods to prevent VRE infection and prevalence. No clonal outbreak happened among 11 VRE.

关 键 词：万古霉素耐药肠球菌 多重PCR 脉冲场凝胶电泳 

分 类 号：R446.5[医药卫生—诊断学]