UPLC-MS/MS联用法分析灯盏花乙素在胃肠道的代谢物  

Study on metabolites of scutellarin in gastrointestinal tract by UPLC-MS/MS method

作　　者：居文政[1,2] 储继红[1] 谭仁祥[2] 熊宁宁[1] 

机构地区：[1]南京中医药大学附属医院临床药理科,江苏-安徽中西药临床药代动力学联合实验室 [2]南京大学生命科学院

出　　处：《中国临床药理学与治疗学》2006年第3期292-295,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：科技部"临床试验关键技术及平台研究"课题资助(№2004AA2Z3766)

摘　　要：目的:研究灯盏花乙素在胃肠道的代谢物。方法:分别用盐酸、β-葡糖醛酸苷酶水解灯盏花乙素,并用健康志愿者的肠内菌对灯盏花乙素进行转化,用超高效液相-串联质谱联用法分析代谢物。结果:灯盏花乙素可被盐酸、β-葡糖醛酸苷酶水解为苷元,可被肠内菌群转化为苷元。健康受试者口服灯盏花乙素后,血浆中能检测到灯盏花乙素苷元。结论:健康受试者口服灯盏花乙素后,在吸收进入血液之前,胃肠道内灯盏花乙素与其苷元并存,而苷元更易于吸收,以总苷元为检测对象研究灯盏花乙素药代动力学是合理的。AIM： The metabolites of scutellarin were studied in gastrointestinal tract. METHODS： Scutellarin was hydrolyzed with hydrochloric acid and β-glucuronidase. Human intestinal flora and scutellarin were incubated in vitro. The metabolites were identified by UPLC-MS/ MS method. RESULTS： Scutellarin could be hydrolyzed with hydrochloric acid and β-glucuronidase, and could be metabolized by human intestinal bacteria. A main metabolite, scutellarein, also was found in the plasma of healthy volunteers after oral administration. CONCLUSION： There were scutellarin and scutellarein in intestinal before absorption. It was suitable for scutellarin pharmacokinetic studies to determine scuteilarein or total aglycone.

关 键 词：灯盏花乙素 超高效液相-串联质谱联用 法 酸水解 β-葡糖醛酸苷酶 肠内菌代谢 

分 类 号：R969[医药卫生—药理学]