匹维溴胺对束缚应激大鼠结肠不同部位和肌层平滑肌条的作用(英文)  

Effect of Pinaverium Bromide on different layers and segments of colonic smooth muscle strips in wrap restraint stress rats

作　　者：李翠萍[1] 刘诗[1] 徐三平[1] 钱伟[1] 侯晓华[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院消化内科

出　　处：《中国临床药理学与治疗学》2006年第3期314-318,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:评价匹维溴胺对束缚应激大鼠离体结肠平滑肌条的影响并探讨其对各型IBS可能的作用机理。方法:成年SD大鼠随机分为模型组和对照组,模型组用自制硬塑胶模具束缚2 h。分别制作两组大鼠近端及远端结肠的环行肌和纵行肌条共64条(每组肌条32个,各肌层8个)。采用离体平滑肌条器官浴槽实验方法,观察不同浓度匹维溴胺对平滑肌条自发性收缩活动的影响。结果:匹维溴胺对模型组和对照组离体结肠平滑肌条自发性收缩活动均表现为抑制作用,但对模型组效应较对照组显著(近端结肠28.54±4.82vs7.48±1.65,21.75±1.00vs12.56±3.15;远端结肠15.71±5.27vs3.89±1.16,20.16±3.16vs7.56±1.96)(P<0.05)。匹维溴胺对近端结肠平滑肌收缩的抑制作用较远端结肠显著(P<0.05)。匹维溴胺对相同部位结肠环行肌与纵行肌的抑制作用无显著性差异(P>0.05)。结论:匹维溴胺能抑制束缚应激大鼠离体结肠平滑肌的收缩,且对近端结肠收缩的抑制作用显著高于远端结肠;但在相同部位,对环行肌和纵行肌收缩的抑制作用无显著性差异。AIM： To evaluate effects of Pinaverium Bromide on different segments and layers of colonic smooth muscle in wrap restraint stress （WRS） rats and explore its possible therapeutic mechanism on different types of irritable bowel syndrome （IBS）. METHODS： Adult SD rats were randomly divided into model group （wrap restraint stress group） and control group. Colonic smooth muscle strips were made from different segments and layers in two groups. The spontaneous contraction activities of colonic longitudinal/circular muscle （LM/CM） strips of rats were observed with organ bath system before and after addition of series concentrations of pinaverium. BESULTS： Pinaverium Bromide caused concentrationdependent inhibition of colonic smooth muscle, the inhibitory effect of pinaverium in model group was significantly stronger than that in control group（proximal colon： 28.54±4.82 vs 7.48± 1.65,21.75± 1.00 vs 12.56±3.15; distal colon： 15.71± 5.27 vs 3.89 ± 1.16, 20.16 ± 3.16 vs 7.56± 1.96 ）（P 〈0.05）. Compared with that of distal colon, inhibitory effect of pinaverium was significantly higher of proximal colon （ P 〈 0.05）. For the inhibition of pinaverium, there was no significant difference between LM and CM strips in the same intestinal segments （P 〉 0.05 ）. CONCLUSION： Effects of Pinaverium Bromide on different colonic muscle layers and segments in WRS rats is probably related with its therapeutic mechanism on different types of IBS.

关 键 词：匹维溴胺 束缚应激 大鼠 结肠平滑肌条 自发性收缩 

分 类 号：R965.2[医药卫生—药理学]