人全厚皮片移植裸鼠模型烧伤后MMP-1、TIMP-1、TGF-β_1的表达  

Expressions of MMP-1, TIMP-1, TGF-β_1 in a nude mouse model of transplanted human skin during the process of burn wound healing and hypertrophic scar forming

作　　者：李钢[1] 李世荣[2] 杨东运[2] 洪宝发[1] 

机构地区：[1]解放军总医院泌尿外科 [2]第三军医大学西南整形美容外科医院,北京100853

出　　处：《中国实用美容整形外科杂志》2006年第2期131-134,共4页

基　　金：国家自然科学基金资助项目(30000175)

摘　　要：目的研究基质金属蛋白酶-1（MMP-1）、金属蛋白酶组织抑制因子-1（TIMP-1）、转移生长因子-1（TGF—β1）在烧伤创面愈合至瘢痕（HS）形成过程中的表达、关系和作用。方法将人的正常全厚皮肤移植于裸鼠，待其完全存活后造成深Ⅱ度烧伤创面。用免疫组织化学染色的方法，观察MMP-1、TIMP-1、TGF—β1在烧伤创面愈合至HS形成全过程中的表达。结果MMP-1于烧伤后第2天出现，第3～15天表达较强，第28天已下降到基底值。TIMP-1于烧伤后第2天出现，一直持续到烧伤后5个月HS形成，其表达类似于MMP-1的接触界面模式，但无MMP-1规则，且在血管周围有明显的分布。TGF—β1于烧伤后12h出现，一直持续到烧伤后5个月HS的形成。三种因子在不同结构的接触界面表达明显。结论在创伤愈合的炎症期和增生期，MMP-1和TIMP-1两种力量处于动态平衡；在重塑期，TIMP-1通过抑制MMP-1的表达减少胶原降解，从而诱导HS形成。TGF—β1在创伤愈合过程中广泛和长期的表达模式，与它对细胞功能广泛的调节作用一致，与它对MMP-1的抑制作用也一致。Objective To explore the interaction and expression of MMP - 1, TIMP - 1, TGF- β1 on burn wound healing and HS forming. Methods Normal human full- thickness skin grafts were transplanted to the back of nude （athyrnic） mice, after these grafts had taken, deep Ⅱ degree bum wounds were made in the middie of these grafts. During the process of bum wound healing and HS formation the expression of MMP - 1, TIMP- 1, TGF- β1 were detected by immunohistochemistry examination. Results Immunoreactive MMP - 1 was first noted by day 2 after burns and became more prominent from day 3 to day 15, and then decreased rapidly. Immunoreactive TIMP- 1 was also observed by day 2 and didn＇t appear untill the HS forming. The expression of the TIMP- 1 had the same interface pattern as described for MMP- 1. Immunostainlng of TIMP - 1 was irregular and discrepant, but was obvious around vascular tissues. Immunoreaetive TGF- β1 could be seen by hour 12 and kept expressiong through the process of wound healing and scar forming. Immunostaining for MMP- 1, TIMP- 1, TGF -β1 was markedly increased at boundary regions marked by connective tissue type. Conclusion The balance between MMP - 1 and TIMP - 1 during the inflammation and granulation phases might be important for the regulation of collagen proteins degradation. Within the remodeling phase, the inhibition of MMP- 1 by TIMP - 1 and the decrease of collagen degradation might lead to HS forming. The widespread and long- term expression of TGF- β1 during the process of bum wound healing and FIS forming not only reveals its comprehensive function but also is coincident with its role in the inhibition of MMP- 1.

关 键 词：MMP-1 TIMP-1 TGF—β1 增生性瘢痕 裸鼠 烧伤 

分 类 号：R644[医药卫生—外科学]