骨髓基质成骨细胞复合支架材料构建组织工程性肌骨瓣的实验研究  

Tissue-engineered muscle pedicel bone graft constructed by in vitro cultured marrow cells combined with HA/TCP

作　　者：徐学武[1] 王锋[1] 郭铁芳[1] 王冬艳[1] 韩雪峰[1] 

机构地区：[1]哈尔滨医科大学附属第二医院骨一科,黑龙江哈尔滨150086

出　　处：《中国实用美容整形外科杂志》2006年第2期138-140,共3页

摘　　要：目的探索用羟基磷灰石/磷酸三钙(HA/TCP)的支架材料与成骨细胞复合构成组织工程性肌骨瓣修复骨缺损的能力。方法自2004年12月至2005年5月,于新西兰大耳白兔髂后上棘抽取骨髓基质干细胞并诱导分化为成骨细胞,然后与HA/TCP结合,共培养2周,通过相差显微镜、扫描电镜观察体外细胞生长情况,最后将复合物埋入兔的背阔肌下构成组织工程性肌骨瓣。术后6周进行大体解剖观察,HE染色,研究组织工程性肌骨瓣的生长情况。结果扫描电镜显示支架材料表面和孔隙内均有成骨细胞生长,有良好的增殖活动性和稳定细胞表型,材料中心区未见细胞生长,植入体内6周后取材见内植物有新骨形成,多位于内植物表面,可见成骨细胞、骨细胞、髓腔样结构、板层样骨基质等正常骨组织结构。结论骨髓来源的成骨细胞可用作骨组织工程的种子细胞,此种组织工程性肌骨瓣生长良好,可成功构建组织工程化骨。Objective To construct a tissue- engineeres muscle pedicel bone graft by culture marrow ceils combined with biodegradable polymeric scoffolds（HA/TCP） which be used to repairing a segmental of long bone. Methods From December 2004 to May 2005, bone marrow stromal ceUs（BMSCS） being aspi- rated from spina iliaca posterior superior of young rabbits were induced into osteoblasts in vitro. Osteoblasts which were isolated from bone marrow stromal ceils of New Zealand rabbits were cultured as the seeding ceils, and the cells were cultured with HA/TCP in vitro. The complex was observed under phase microscope and histology to evaluate the interaction between osteoblasts and HA/TCP. The compund co - cultured for 14 days was implanted intermuscularly into muscle latissimus dorsi of rabbits which were investigated at 6 weeks after postoperatively. Investigation of the muscle pedicel bone graft by means of gross observation, histology of HE. Results Scanning electronic micrographa showed that the pores of HA/TCP and its surface were fiUde with cultured osteoblasts, especially in the longer cultured block, no cell growth was found in the central area, new bone formation was observed in all blocks at 6 weeks after implantation. Conclusion The osteoblasts froth bone marrow stromal cells can be used as the seeded cells for hone tissue engineering, Bio - derived materials being constituted with BMSCS may be a good option in cosntruction of bone tissue engineering.

关 键 词：骨髓基质干细胞 羟基磷灰石/磷酸三钙 肌骨瓣 组织工程 

分 类 号：R687.3[医药卫生—骨科学]