血管内皮生长因子在人胎盘绒毛滋养层表达与绒毛血管生成  

Expression of vascular endothelial growth factor in human placental trophoblasts and angiogenesis in the chorionic villi

作　　者：贾建军[1] 王自能[1] 罗新[1] 陈新[1] 

机构地区：[1]暨南大学附属第一医院妇产科,广东广州510632

出　　处：《南方医科大学学报》2006年第4期505-508,共4页Journal of Southern Medical University

摘　　要：目的确定血管内皮生长因子(VEGF)在人胎盘绒毛滋养层的表达,探讨VEGF在绒毛间质血管生成中的作用。方法取孕6-9周行人工流产术的胎盘10例、孕18-22周行水囊引产术的胎盘(10例)、孕37-38周行剖宫产的胎盘(10 例),分3组进行免疫组织化学染色与定量分析并对绒毛血管行体视学研究。结果在3组中,VEGF在绒毛滋养层的表达有显著性差异(28．19±3．01,18．65±2．43,4．95±0．86,P<0．01),绒毛间质血管的管径短轴参数无显著性变化(26．67±7．74, 25．08±4．67,23．36±5．30,P>0．05),血管的长度密度呈显著性增加(1．46±0．64,5．58±1．31,19．56±1．40,P<0．01)。结论随着孕期发展,VEGF在绒毛滋养层的表达逐渐减弱,但血管长度密度却增加,说明VEGF并非调控绒毛血管生成的唯一因子。Objective To investigate the expression of vascular endothelial growth factor （VEGF） in human placental trophoblasts and the role of VEGF in regulating placental villous angiogenesis. Methods Placental samples were obtained from 10 pregnant women receiving induced abortion in the first trimester, 10 receiving induced labor in the second trimester and 10 having cesarean section at term delivery, with gestational duration of 6-9, 18-22 and 37-38 weeks, respectively. All the samples were fixed in formalin solution and prepared for the morphological study. The expression of VEGF and vascular distribution in the placental villi were examined and evaluated by immunohistochemistry and stereomorphometry, respectively. Results In the course ofpreguancy, there was a significant decrease in the level of VEGF expression in chorionic villi （28.19±3.01, 18.65±2.43, 4.95±0.86, respectively, P〈0.0 1）. The radial parameters of the blood vessels showed no significant changes （26.67±7.74, 25.08±4.67, 23.36 ±5.30, respectively, P〉0.05）, but the length density of the blood vessels increased significantly （1.46 ±0.64, 5.58 ± 1.3 1, 19.56 _± 1.40, respectively, P〈0.01 ）. Conclusion During gestation, VEGF expression in chorionic villi gradually weakens but the length density of the blood vessels increases, indicating that VEGF is not the only regulatory factor of angiogenesis in the chorionic villi.

关 键 词：血管内皮生长因子 滋养层细胞 绒毛血管 血管生成 

分 类 号：R714[医药卫生—妇产科学]