胸腺细胞凋亡的死亡信号表达  

Expression of death signal pathway in thymocyte apoptosis

作　　者：周瑞祥[1] 黄云梅[1] 刘卉[1] 魏建恩[1] 谢美蓉[1] 林静[1] 林建银[2] 

机构地区：[1]福建医科大学人体解剖学与组织胚胎学系 [2]福建医科大学分子医学研究中心,福州350004

出　　处：《解剖学杂志》2006年第2期171-173,212,共4页Chinese Journal of Anatomy

基　　金：福建省自然科学基金(C0410028)

摘　　要：目的:探讨胸腺细胞凋亡过程中死亡信号通路的存在依据及其意义。方法:选用2月龄清洁级SD大鼠,用ABC法检测胸腺细胞Fas/FasL、Caspase-3、Caspase-8、Caspase-12表达,用RT-PCR法检测培养胸腺细胞的TNF-β和Caspase-3表达。结果:Fas在胸腺皮、髓质细胞弥散均匀分布,FasL阳性细胞较少,Caspase-3主要分布于皮质胸腺细胞,Caspase-8则分布于胸腺髓质,Caspase-12在皮、髓质胸腺细胞均匀分布,RT-PCR检测显示胸腺细胞均表达TNF-β和Caspase-3。结论:大鼠胸腺内存在完整的细胞凋亡死亡信号通路的各种成分。死亡信号通路在胸腺细胞凋亡过程中可能扮演重要角色。Objective： To study the expression and physiological action of death signal pathway in thymocyte apoptosis in rats. Methods： The Fas/FasL, Caspase-3, Caspase-8 and Caspase-12 positive cells in SD rat thymus were dyed with immunocytochemical ABC methods. TNF-β and Caspase-3 expression in rat thymocyte was detected by RT-PCR Results： Fas positive thymocytes were present in both cortex and medulla. Caspase-3 positive cells were distributed mainly in cortex and Caspase-8 in medulla. Caspase-12 expressed evenly in cortex and medulla. Both TNF-β and Caspase-3 were expressed in rat thymocytes. Conclusion： There are full compositions of death signal pathway in rat thymus, which may play an important role in the thymocyte apoptosis.

关 键 词：胸腺 细胞凋亡 FAS/FASL 半胱天冬酶 

分 类 号：R392[医药卫生—免疫学]