大鼠海马γ-氨基丁酸-A受体在生后发育中的表达  

Expression of GABA-A receptor in rat hippocampus during postnatal development

作　　者：王晓亚[1] 王增贤[1] 王怀经[2] 李振中[2] 

机构地区：[1]泰山医学院基础医学研究所,泰安271000 [2]山东大学医学院

出　　处：《解剖学杂志》2006年第2期200-202,208,共4页Chinese Journal of Anatomy

摘　　要：目的:研究大鼠生后发育过程中海马组织γ-氨基丁酸-A(GABA-A)受体的表达规律。方法:用免疫组织化学和PCR技术,检测不同年龄大鼠海马组织GABA-A受体及编码该受体的mRNA表达,并用图像分析方法进行定量研究。结果:大鼠海马组织在生后3 d已经出现GABA-A受体免疫反应,以后逐渐增强,到生后30 d达到最高值,海马各区GABA-A受体免疫反应强度没有显著的差别;编码GABA-A受体的mRNA表达也有类似的增龄性增多,但其表达高峰值提前到14 d。结论:生后大鼠海马GABA-A受体表达在一定时期内呈增龄性表达增强的趋势。Objective： To study the expression of GABA-A receptor in rat hippocampus during postnatal development. Methods： Immunohistochemistry, PCR technique and the image analysis were used to study the expression of GABA-A receptor mRNA at various stages of the development. Results： GABA-A receptor was expressed early on the third postnatal day and increased gradually to its peak around the 30th day. There was no statistical difference in GABA-A receptor expression between various hippocampal areas. The expression of GABA-A receptor mRNA increased with age, but the peak of mRNA expression was on 14th day, which was earlier than that of the receptor. Conclusion： During the postnatal development, the expression of GABA-A receptor and its mRNA tend to increase with the rat aging.

关 键 词：海马 GABA—A受体 发育 大鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]