5-羟色胺转运体在酗酒人脑干头侧中缝核群的表达  

Expression of serotonin transporter in rostral raphe nuclei of brainstem in alcoholic human

作　　者：石葛明[1] 李双成[2] 崔慧先[2] 石雄[3] 樊平[2] 曹翠丽[1] 王志红[4] 

机构地区：[1]河北医科大学神经生物研究室,石家庄050017 [2]河北医科大学解剖学教研室 [3]唐山市第八中学生物组 [4]河北医科大学附属二院神经内科

出　　处：《解剖学杂志》2006年第2期213-215,219,共4页Chinese Journal of Anatomy

摘　　要：目的:观察酗酒人脑标本脑干头侧中缝核群5-羟色胺转运体(SERT)的表达变化。方法:应用免疫放射自显影的方法,显示SERT免疫反应强度在酗酒人脑标本脑干头侧中缝核群的变化,并与健康人脑标本比较。结果:健康组SERT免疫反应强标记信号在脑桥头侧和中脑主要集中分布在与中缝核群相同的区域。在酗酒人脑标本,正常分布于脑干头侧中缝核群的SERT免疫反应标记信号减弱;中缝正中核、中缝背核尾侧部、中缝背核束间部、中缝背核腹侧部、中缝背核背侧部的SERT免疫反应含量强度与健康组相应区域比较显著降低。结论:酗酒者头侧中缝核群SERT蛋白表达降低。Objective： To study the expression changes of serotonin transporter （SERT） in the rostral raphe nuclei of postmortem brainstern in alcoholic human. Methods： Immunoautoradiography method was used to reveal SERT immunoreactive intensity and the results were compared with those of healthy controls. Results： Strongly labeling signals of SERF were mainly found in the rostral raphe nuclei of the rostral pons and rnidbrain in control group, while the signal was reduced in those nuclei of alcoholic group. Quantitative analysis revealed that the intensity of SERT immunoreaetivity （SERT-IR） was significantly lower in median raphe nucleus （MnR）, caudal part of dorsal raphe nucleus （DRC）, interfaseieular part of dorsal raphe nucleus （DRI）, ventral part of dorsal raphe nucleus （DRV） and dorsal part of dorsal raphe nucleus （DRD） of alcoholic group compared with that in corresponding nuclei of control group. The difference of SERT-IR intensity in those nuclei of both group was statistically signifi- cant （P〈0.05： MnR, DRV and DRD; P〈0. 01： DRI and DRC respectively）. Conclusion： Reduced expression of the serotonin transporter in the rostral raphe nuclei is found in the alcoholic human.

关 键 词：酗酒 5-羟色胺转运体 中缝核 免疫放射自显影 人脑 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]