白眉蝮蛇降纤酶的纯化及特性分析  

Purification and characterization of defibrase from Agkistrodon ussuriensis emellaov snake venom

作　　者：魏金荣[1] 张玉魁[2] 张岐山[3] 

机构地区：[1]中国医科大学基础医学院生物化学与分子生物学教研室,辽宁沈阳110001 [2]中国医科大学生物芯片中心,辽宁沈阳110001 [3]中国医科大学生物技术研究所,辽宁沈阳110001

出　　处：《中国生化药物杂志》2006年第2期101-103,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的从白眉蝮蛇蛇毒中提取降纤酶,并对其进行特性分析。方法采用一步亲和色谱法分离纯化降纤酶,用高效液相色谱分析其纯度,SDS-PAGE测定其相对分子质量。结果分离得到的降纤酶纯度高(99%以上),SDS-PAGE分析为一条带,相对分子质量为36 000,比活性为4 800 u/mg。结论用一步亲和色谱法从白眉蝮蛇蛇毒中纯化降纤酶的方法简单有效,所得产品纯度高、产量高。Purpose To characterize defibrase enzyme isolated from the venom of Agkistrodon ussuriensis emellaov. Methods Defibrase was purified by one-step affinity chromatographic method, and the purified enzyme was analyzed by HPLC and SDS-PAGE, respectively. Results The enzyme was purified with a high yield and high purity. SDS-PAGE data indicated that the purified enzyme was a single band, and its molecular weight was about 36 000. The specific enzymatic activity was measured as 4 800 u/mg. Conclusion The method for purifying defibrase by one-step affinity chromatographic method is convenient and efficient.

关 键 词：白眉蝮蛇 降纤酶 纯化 

分 类 号：R282.74[医药卫生—中药学] R996.3[医药卫生—中医学]