AGM区基质细胞促进小鼠胚胎干细胞向成血-血管干细胞分化的实验研究  

Aorta-gonad-mesonephros Region-derived Stromal Cells Promoting the Differentiation of Mouse Embryonic Stem Cells into Hemangioblasts

作　　者：付劲蓉[1] 刘文励[1] 周剑锋[1] 孙汉英[1] 冉丹[1] 郑邈[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院血液科,武汉430030

出　　处：《华中科技大学学报（医学版）》2006年第2期159-162,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30570773);中国博士后科学基金资助项目(No.2004036440)

摘　　要：目的探讨主动脉-性腺-中肾(AGM)区来源的基质细胞诱导胚胎干细胞(ESC)向成血-血管干细胞分化的促进作用。方法从孕11 d的小鼠胚胎AGM区分离、培养基质细胞,鉴定后用作支持细胞,与小鼠胚胎干细胞共同培养,通过原始细胞集落(BL-CFC)的形成情况及流式细胞仪检测CD34+/Flt-1+细胞比例,评价AGM区基质细胞对小鼠ESC-D3细胞系向成血-血管干细胞的定向诱导作用。结果在AGM区基质细胞的支持下,由ESC来源的CD34+细胞由(12.71±1.76)%增加到(39.71±3.76)%,并且CD34+/Flt-1+细胞比例明显提高,达到(26.59±1.77)%;同时由ESC来源的代表成血-血管干细胞的BL-CFC集落增加了3倍。结论AGM区基质细胞可促进胚胎干细胞向成血-血管干细胞分化,探明其机制对研究造血及血管发育机制有积极意义。Objective To explore the role of aorta-gonad-mesonephros （AGM）-derived stromal cells in promoting the differentiation of embryonic stem cells （ESCs） into hemangioblasts. Methods The murine stromal cells were separated and cultured from the AGM region of a 11 daypostcoitum （dpc） mouse embryo. After phenotypic analysis, the stromal cells were cocultured with the ESCs. ESC-derived and cytokine-induced hemangioblasts were detected by flow cytometry and colony cells as say. Results Supported by AGM-derived stromal cells, the ratio of ESCs-derived CD34^＋ was increased from （12.71±1.76） % to （39.71±3.76） %, and CD34^＋/Flt-1^＋ cells were markedly increased to （26.59± 1.77） %. The number of BL-CFCs produced by the hematopoietic precursor cells induced by co-cuhure with AGM stromal cells was increased by 3 times as compared with the control cells. Conclusion AGM-derived stromal cells, which could promote the differentiation of ESCs into hemangioblasts, play an active role both in hematopolesis and angiogenesis.

关 键 词：胚胎干细胞 成血血管干细胞 主动脉性腺-中肾 造血发育 血管发育 

分 类 号：R55[医药卫生—血液循环系统疾病]