肝炎平对血吸虫性肝纤维化家兔肝组织HGF和TGF-β_1表达的影响  

Effect of Ganyanping on HGF and TGF-β_1 in Rabbit with Shchistosomal Liver Fibrosis

作　　者：晏维[1] 唐望先[1] 付妤 张芳杰 金琦 

机构地区：[1]华中科技大学同济医学院附属同济医院肝病研究所,武汉430030

出　　处：《华中科技大学学报（医学版）》2006年第2期199-201,205,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30440088)

摘　　要：目的观察肝炎平对日本血吸虫感染致肝纤维化家兔肝组织肝细胞生长因子(HGF)和转化生长因子β1(TGF-β1)表达的影响。方法实验分为3组:正常对照组、模型组及肝炎平治疗组。模型组及肝炎平治疗组通过腹部贴片法感染日本血吸虫(180条/只),6周后用吡喹酮200 mg/d,整片喂服,共2 d,进行杀虫治疗,杀虫治疗后肝炎平治疗组予以肝炎平治疗6周。苏木精-伊红染色观察肝脏病理形态改变。免疫组化法检测各组HGF、TGF-β1在肝内的表达;RT-PCR和Western blot法分别检测各组HGF、TGF-β1mRNA和蛋白表达水平。结果血吸虫性肝纤维化家兔经肝炎平治疗后肝组织内TGF-β1的蛋白和mRNA表达降低,而HGF的表达增强,与模型组比较差异有显著性意义(均P<0.05)。结论肝炎平抑制血吸虫性肝纤维化家兔肝组织TGF-β1的表达,促进HGF的表达,这可能是其抗血吸虫性肝纤维化作用的机制之一。Objective To study the effect of Ganyanping on transforming growth factor-β1 （TGF-β1） and hepatoeyte growth factor （HGF） of rabbit model of schistosmal liver fibrosis. Methods The rabbits were separated randomly into three groups： normal control group（N）, model group（M） and Ganyanping treated group（E） those in. The rabbits in groups M and E were infected with cercariae of Schistosomiasis japonicum （180 cercariae per rabbit） and those in group E treated with Ganyanping for 6 weeks after the model was made successfully. The effect of Ganyanping was explored by the expressions and sites of HGF and TGF-β1 in liver tissues by immunohistochemical staining. The protein levels of HGF and TGF-β1 were analyzed by Western blotting. RT PCR was used to analysis the mRNA level of HGF and TGF-β1. Results Compared with the group M, the espression of HGF was increased,while the expression of TGF-β1 decreased in the liver in the group E. The difference was signifieant（P〈0.05）. Conclusion Ganyanping could inhibit the expression of TGF-β1 and enhance the expression of HGF in the liver of rabbit with schistosomal liver fibrosis, which may be one of the antifibrotic mechanism of Ganyanping.

关 键 词：肝炎平 肝细胞生长因子 转化生长因子β1 血吸虫 肝纤维化 

分 类 号：Q291[生物学—细胞生物学]